...sessions and reported the intel back to my shyer classmates. For my undergraduate degree, I majored in human...stigma around sexual and reproductive health issues creates conditions under which misinformation can flourish...anxious, whispered questions from friends and classmates back in high school, and it’s oddly fitting that...to read short abstracts comparing the mortality rates between two controversial health issues. One group... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas...

...diseases, and several treatments are currently being tested in clinical trials. It is speeding the process ...cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the...CFTR) by homologous recombination in cultured intestinal stem cells of patients with cystic fibrosis (...also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical for... "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients...

...TCSs). TCSs consist of a histidine kinase that phosphorylates a response regulator (RR). TCS-induced responses...far-red light or dark conditions, the chromophore activates the RR (OmpR) via phosphorylation, promoting transcription...below). Subsequent exposure to red light rapidly deactivates the system. Voigt’s lab used this system to develop...far-red light or dark conditions, the chromophore activates the response regulator via phosphorylation, promoting...reporter. Subsequent exposure to red light rapidly deactivates the system. Version 1 of this far-red/red light-responsive...

...your resume? How can you stand out among all the candidates applying for the position?   Here are a few things...people choose a reversed chronological style (the latest to the earliest) or a functional one. Pick one ...surprised to learn how often I see resumes from good candidates that I almost missed because I had to “dig” hard...topic will give you some advantage over other candidates. Write about your transferable skills Some of...career services offices. You might think only undergraduates use these services, but some of the general...

...interactions. It also can’t recognize PAMs, so it associates with random DNA sequences (Sternberg et al., ...sequencines. This helps explain why Cas9 can’t locate PAM sites until after RNA loading: the structure to interrogate...for complementarity with its guide RNA. But how to test DNA bases that are already snugly paired to each.... As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after perfect...conformations. This allows it to unwind the DNA and test for sequence complementarity with its guide RNA....California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber...

...randomly, while others use attP sequence docking sites so that you can control exactly in which locus your...different loci. Fortunately, there are many docking sites available for integration. 2. How soon would you... development, organs of interest, such as the intestines, can be dissected at various times throughout... resources of the Drosophila community to easily test your hypotheses. Many thanks to our guest blogger... H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila...

...after this initial launch so stay tuned for more updates. Overview of SnapGene powered plasmid maps at...be detected by our in-house software and also eliminates many of the extraneous ORFs that cluttered our...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you to dive...above and their functions. We hope that these updates will make it much easier to browse the repository...improvements. Let us know what you think about the updates in the comments section below and keep checking...

...perfect. They may be slow, can only target certain sites, or make only a subset of base substitutions.  ...better base editors with the help of phages! The latest ABE from the Liu lab is ABE7.10 which contains ...the phage is not infectious. The editing speed dictates which phage variants can propagate faster than...compared to the original TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e...all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...

...rather than a perfect lesson plan or outstanding test scores.  As a result, Kuldell launched the BioBuilder... fields rely on rapid prototyping to develop and test hypotheses. Students then use cellular extracts ...means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech collaboration has resulted...classroom poster — with both of these resources road tested over the summer with dozens of teachers around ...approaches and scientific know-how to design/build/test their own project ideas using synthetic biology....

... it is standard practice to compare degradation rates between samples treated with or without an autophagy...ATG) in yeast. Atg8p (“p” stands for protein) associates with autophagosomal membranes, so tagging it ...monitor autophagy, but under some conditions it aggregates in an autophagy-independent manner. Additionally...induce autophagy, red but not green fluorescence accumulates in the vacuole. See Figure 2 for an example of...Autophagy. Methods in Enzymology Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A, 33-42. doi...

...Monitor neo-substrates Small molecule degraders may recruit CRBN to nonstandard or “neo-substrates.” We advise...interest prior to starting knock-in experiments. Test dTAG molecule toxicity in new cell lines Treat parental...evaluation of degradation of known cereblon neo-substrates (IKZF1, IKZF3, GSPT1, ZFP91, and CK1a). While...While expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3...

...catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...biosensors or biosensors that detect new redox metabolites are vital for researchers to better understand...mechanical allodynia, a condition where a light touch creates a painful sensation. The dorsal horn of the spinal...perceiving touch inputs. In this system, light activates the neurons (via oChIEF) and a chemogenetic agonist...identify the mutants for further enrichment. Tian notes that this platform can be used to engineer many ...

...functions due to mutations accumulated over time? Undergraduates can help to answer this question! The Pathways...can be relatively confident that the gene from the test species has a similar function to the gene in the...enzymatic steps (5). In their experiments, students test whether or not proteins from the fission yeast, ...Excel: Producing One Million Additional College Graduates with Degrees in Science, Technology, Engineering...PMCID: PMC4012490. 4. Hoffman, C.S., Wood, V., & Fantes, P.A. An ancient yeast for young geneticists: A...

...They will come in handy here! Crossing your F1 heterozygotes to each other will generate approximately 1 ...wild type at one or both of the alleles, or heterozygotes, are not a waste. Best practice is to use littermate...transgenic strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...chromosomes recombine during meiosis, creating gametes that contain a mix of genetic material from each...recombination at the floxed site, your control should be littermates that express Cre but not loxP. With this two-...

... to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection... systems. What’s the error rate? The Doench lab tested the Fragmid system by sequencing 60 individual ... assemblies created over five months. When they tested those clones (in duplicate), 93% had the correct...your backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate...References McGee, A. V., Liu, Y. V., Griffith, A. L., Szegletes, Z. M., Wen, B., Kraus, C., Miller, N. W., Steger...

...A4 and A8-A10. The researchers selected 8 of the tested ABE8 constructs (ABE8.8-m/d, ABE8.13-m/d, ABE8.17...2 guanines). This limits the use of ABEs as DNA sites that do not contain a suitable PAM cannot be edited...for primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently... gRNA used (Gaudelli et al., 2017). The authors tested ABE8.17-m and found that one base change mutation...overall improved base editing capacity, even at sites that used to be difficult to target. In particular...

...review analyzing and explaining the antibody crisis states that up to 50% of all commercially available antibodies...to more complex brain systems such as non-human primates and humans. Since we need larger-volume antibodies...extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and provide general...includes accuracy, strength, and reproducibility. We test the antibodies on human and mouse tissue and compare... distinct imaged pattern, and exposure times. To test the antibody’s reproducibility, each antibody is...

...characterized by misfolding of the TTR protein, which contributes to the disease phenotype. Intellia initiated ...Biosciences is currently running this trial, with more updates expected in the coming months/years. The CRISPR-HIV... (Hussein et al). It employs Cas9 to cut at two sites within the viral genome to excise a large portion...disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies... "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients...

...it ranks guides by CFD score, and it mutates HDR donor templates to avoid re-cutting when needed. It gives...each gene, we needed to design guide RNAs, donor templates and sequencing primers for quality control. At...sophisticated features of CrispyCrunch is how it mutates guide sequences to prevent re-cutting of the sequence... the above are options, the algorithm silently mutates codons in the guide sequence, one by one, from ...

...surprise and delight,” the very first protein they tested — E. coli expressing good ol’ EGFP — showed a slight...and Maria were skeptical. The team was careful to test the possibility that this might just be an artifact...area measures approximately 82 mm across. Video courtesy of Andrew York and Maria Ingaramo.   MagLOV shows...tools.  Embracing science in the open The York Lab operates with a strong commitment to exploration and collaboration...self-publishing directly on his website (and often posting updates on social media) and freely sharing code, raw data...