Antibodies 101: Designing Your First Flow Panel
Type
Blog Post
Published
May 28, 2024, 1:45 p.m.
...that is, let's look at Figure 2. The blue laser excites at a wavelength of 488 nm (Table 1), which is near...line, Figure 2). The yellow/green laser, however, excites at 561 nm, which is close to the global maximum...more photons are absorbed and then emitted, which creates a relatively stronger — or brighter — signal.
...the same laser type. Knowing that the red laser excites at 640 nm (Table 1), while comparing the two fluorophore's...is about 750 nm. If we were to use a laser that excites at 750 nm, APC-Cy7 would show a much brighter signal...absorbed and emitted by matter, and how this process creates visible colors. Next, you learned about how fluorophores...