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... Addgene has tested these capsid plasmids in vitro and uses them in our own AAV production processes...for neuronal tracing. Labeling was nontoxic and activity-dependent, with more labeling across active synapses...

... and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed...Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be modified to assay...

...preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards ...cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all three germ layers, form ...

...Golden Gate assembly involves the simultaneous activities of a Type IIS restriction enzyme such as BsaI...base pair substrates, it demonstrates significant activity on some mismatch-containing pairings. During Golden...

...of interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some...

...bead assay (Figure 2). After finding no cross-reactivity, antibody candidates underwent affinity screening... that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library...

... proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection of synthetic...selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each mRNA molecule...

...excess energy at wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths...non-specific binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination...

...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at...CRISPR experiments. It has certainly boosted our productivity in the Biohub. We hope it will do the same for...

...conformational changes in the antigen or lower detection sensitivity of the assay. Where possible, it can be useful...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....

...more challenging to design a hands-on interactive activity using elephants than it is to design one demonstrating...or do you need a full exhibit? In a five minute activity at a street fair, or a semester-long after school...

...figures 4A-C). And since they have limited cross-reactivity between different IgG subclasses and different...State University Use Nanobodies to Control Protein Activity Learn more about protein tags Read about E. coli...

... but in less time. The authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours... faster, cheaper, and had a similar level of sensitivity. Figure 2: Steps to purify DNA using a ...

...protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env,... particles with the VSV-G envelope have high infectivity in a wide range of cell types, so they’re ideal...

...efficient polymerase activity, whereas some ratios result in significantly reduced activity (4). To ensure ...

...expressed as fusions that retain their individual activities (1). Researchers also discovered the modular ...activation (TA) domains retain their individual activities when separated (2), and that DBD and TAs from...

...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...it's fun to be involved in these professional activities because I can contribute by, for example, helping...

...the nucleus where they exert their recombinase activity (Logie and Stewart 1995; Metzger et al. 1995).... to compare neuronal morphology and synaptic connectivity before and after the gene knockout in the same...

...system to identify and enrich for base-editing activity The reporters mentioned above all rely on GFP ...reporter system to identify and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https:/...

... situ. As a result, they had to be expressed in vitro before the bacteria were injected, limiting their... Use a subset of the cells to verify the mARG positivity, which can be observed under phase contrast microscopy...