We narrowed to 535 results for: epo
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TypeBlog PostPublishedMay 28, 2019, 12:49 p.m....If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...
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Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m....Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place... complexes. John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m.... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m....allowed us to handle and distribute such a large deposit. Once the project is complete, Ginkgo will have...-19. But, we can’t do this alone. If you have deposited plasmids at Addgene and would like to make them... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....scientists on the team ensure the deposits we received are what the depositing labs intended. With the use ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m.... yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....capstone project report and presentation, during the final week at Addgene. This report shared my work ... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....stock. All of our proceeds go into running the repository and serving scientists better. That includes ...reagents Addgene makes it easy for scientists to deposit and request plasmids (via web interface, processes... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....traditional publications. By submitting your data to a repository (Figshare is currently supported by ReFigure ... about Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular Biology... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....webpage for any specific recommendations from the depositing lab. If your vector is large (>10 kb) or has ...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m.... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...robust immune response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...Resources Learn More about Viral Vectors Browse the Repository ... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx...