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We narrowed to 567 results for: esp

Showing: 241 - 260 of 567 results
  1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... on popular support, which in turn depends on widespread trust in scientists to develop new technologies...trusted to handle such a powerful technology responsibly. An accidental release would be particularly ...
  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...
  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...professional risk involved in setting up a CURE, especially if it’s a new approach within their department...
  4. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...Infographics Low Combines both words and images; especially useful for conveying and contextualizing data...except for space) Real-time audience engagement and response. High connection levels and interaction. Can bring...
  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... figure organization with a different person responsible for each figure. Others still are more casual...focus and critical mass of participants. This is especially true of journal clubs where the very point is...
  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase, so cells stay...Methods favoring homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int...
  7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons have been...Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-guided DNA insertion with CRISPR-associated...
  8. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern similar...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...
  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic...small molecules that do not generate an immune response. Aptamers can be selected for these small ligands...
  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...
  11. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...
  12. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...
  13. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...
  14. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...also very important in a non-research context, especially when considering small to mid-sized companies...When you reach out as a student, people tend to respond in a supportive way and are willing to help because...
  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...Converts A to Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, ..., or C base editors Excises G, T, or C, respectively, to be replaced with another base. References ...
  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...shipping. However, there were issues that persisted despite our best efforts. With a little over half of our...time when a picture is worth a thousand words, especially when it comes to protocols. In 2013, we launched...
  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...counterparts. Those familiar with viral vectors, and especially those familiar with adeno-associated viruses ...chemogenetic tools: protein tools that are activated, respectively, by substrates, light, or engineered ligands...
  18. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...procedure is longer than alternative ELISA methods, especially when secondary antibodies are used.  Competitive...positives.  If testing a recombinant protein, especially one with tags, be sure to include a sample that...
Showing: 241 - 260 of 567 results