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We narrowed to 256 results for: human dat

Showing: 241 - 256 of 256 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https:...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR... of DNA. However, several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to...targeted delivery of Tet1 alone, or coupled with DNA oxidation and repair factors that allow for increased gene...Modification   Leah Schwiesow contributed to updating this article. References Haldeman JM, Conway AE...Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications...

  2. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...strains of E. coli, some of which are deadly to humans. The majority of all common, commercial lab strains... This post was updated on Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...3,300-fold in Drosophila cells and ~24,000-fold in human HeLa cells as compared to basal expression. Co-expression...originally written by Mary Gearing in 2018, and updated by Hannah Dotson in Dec. 2022.  References and...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... to apply to study a Master of Science (MSc) in Human Molecular Genetics to give me an edge over other... in Spanish or French, and so they were ideal candidates for these positions.  I had to reassess what ...lab, but I really struggled with the large scale data analysis, as this is increasingly reliant on programming... required a scientist for a 6 month project to validate some genomic changes, so this arrangement was ...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...researcher at Harvard Medical School working on human artificial chromosomes. Montreal, QC, Canada 45.5017...defend an agenda that halts current data collection, destroys existing data, and guts future funding.I marched...hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear Science and Engineering at Rensselaer...importance.  I think many of us grew to assume that the data might speak for itself but we're now in a rift where...including global warming skeptics – insisted that more data and research funding are critical to averting a ... society, need to: 1) use more citations for out data so our sources are readily available to people who...first step, and continue this work by voting for candidates that respect science, learning to communicate...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286(12):10017-26...fluorescence (CGE-LIF) for detection and can provide data on the quantity of various topologies and multimers...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...developed a self-labeling fusion tag based on the human DNA repair protein AGT that gets covalently linked...(Figure 3). And using self-labeling tags makes updating your labels easy! Instead of taking the time to...

  8. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...body of highly specialized knowledge in a field of human endeavor including but not limited to biotechnology...important for candidates during a job search and it is even more critical for international candidates. Being...international candidates that they should join their relevant associations/groups. These candidates should also...performs in-depth vetting of a candidate’s background to ensure that each candidate meets the requirements set...development, business development, consulting, policy, big data and the list goes on and on. As you think about ...career. It is more likely that an international candidate will be hired for a role that is closely aligned...research role, for a Ph.D. trained scientist. If the candidate is ultimately looking for a role in a different...

  9. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...editors for inducing targeted DNA transversions in human cells. Nature Biotechnology, 39(1), 41–46. https...generated similar deletions at a lower frequency. Their data suggest that DSBs are a common intermediate of CGBE...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... Rinehart lab synthesized the activated form of human mitogen-activated ERK activating kinase 1 (MEK1)...Updated mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab...only the elements needed for function. The lab validated this vector, called pBAM1, by conducting random...Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas resources at www.addgene.org/CRISPR/...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...occurs during Timer protein life due to a form of oxidation (dehydrogenation). MitoTimers are composed of ... the balance of biogenesis and mitochondrial degradation in vitro and in vivo. Merged green and red ...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with plants...quickly screen and investigate transcription factor candidates and the regulation of genes in the biosynthetic...Often a FP is fused to a protein of interest to elucidate the subcellular localization of your protein of...transformations are a great help in screening candidate genes and speeding up research. Certainly, there...Northeastern University. Samuel Mortensen is a PhD candidate at Northeastern University in Boston, MA. He obtained...agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-50. PubMed PMID: 19183892. De Ruijter...agroinfiltration of leaves." Arabidopsis Protocols. Humana Press, 2006. 225-229. PubMed PMID: 16739580. Li...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...attended a very interesting talk by Prof. Erin M Schuman, a former Caltech researcher, now Director at the...newly synthesized proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical...the turnover of one specific protein. Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended...interaction. To study protein levels in situ, the Schumann lab used a modified version of PLA where one antibody...

  13. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing the PCR product...to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA...polynucleotide synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. ...

  14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...through 3 major steps to select for optimal sybody candidates. Figure 2: Outline of the process for selection...display mRNA from the enriched pool of sybody candidates are then reverse transcribed into cDNA fragments...library consisting of phages that display the candidate sybodies in their coat protein to screen for binding...-tag and a His6-tag to the end of each sybody candidate. These tags are used in a final enrichment step... narrowed-down pool (usually 10-30) of sybody candidates are sequenced for identification. All unique ...Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-...

  15. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ...collection in your experiments and published your data, please fill out this form and we’ll add your citation...the following day. Nothing like waking up to fresh data! These same plasmids are then added into our normal...Spike Proteins. In: Methods in Molecular Biology. Humana Press, pp 331–338 https://doi.org/10.1007/978-1...

  16. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...delivery.  Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given...plex with two barcodes per cell. The researchers validated their work with the “signalome,” a 12-plex mixed-subclone...
Showing: 241 - 256 of 256 results