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We narrowed to 383 results for: plasmid dna

Showing: 241 - 260 of 383 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence...preamplification was able to detect as low as 1.2 copies DNA targets and 4.6 copies RNA targets in a 40 min incubation...sensitive to the presence of mismatches within the DNA and the positions of these mismatches. To detect ...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...indiscriminate ssDNA cleavage activity which cleaves the supplied fluorescently labelled ssDNA substrates....

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...Cas9 nuclease, so there’s no potential for aberrant DNA cleavage. Direct HIV-1 cleavage allows T-cells to...level of protein structure, CRISPR gRNAs require DNA sequence-specific binding. Patients’ HIV-1 genomes...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...Rice CM, Bhatia SN. CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus. Sci Rep...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

  3. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ... we use SnapGene for plasmid quality control are: To identify important plasmid features To align NGS...design experiments for further plasmid verification Identifying plasmid features While our quality control...control process for incoming plasmids can vary based on the contents of a plasmid, the first step is always...always to send prepped plasmid DNA for next generation sequencing. We then copy the contents of the resulting...send the plasmid off for Sanger sequencing.     If a repetitive region is present in the plasmid, we might...sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps and sequences...the Addgene Blog How to verify your plasmid Check out our Plasmids 101 featured topic page Additioinal...

  4. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...Heidelberg University. “What is different is the plasmids you use to bring this into your target organism...stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically reducing the time...

  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...changes we’ve made: New CRISPR plasmid pages We’ve given our CRISPR Plasmids and Resources a fresh new design...design, sorting the plasmids we have available by category. Each plasmid page now has a helpful graphic...introduce you to the plasmid class covered on that page. To help you find popular plasmids, we’ve also added... new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids allow...edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids for mammalian...revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that’s not our only job - we strive to also...up with new developments (and, of course, find plasmids that will be useful to you.) With these goals...

  6. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...Addgene. I am currently working on the QC/QA team as a DNA Sequencing Intern. The application process When ... the submitted sequences were a concern to the plasmid function. The steps of the training process gave...very approachable.     Fig. 1: Examples of DNA sequencing QC work    In addition to my responsibilities...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...want delivered to your target cells. This delivered DNA/RNA can then be either permanently integrated into...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...the virus are now delivered in other forms (mRNA, DNA, etc.) to raise immunity. Viral vectors are being...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...macromolecules used in scientific research such as DNA or protein. It comes as no surprise then that stability...Base modifications What is more stable than RNA? DNA! One way to make your gRNA more efficient on a budget...ribonucleotides for deoxynucleotides. A partially DNA gRNA is surprisingly well-tolerated by the Cas9 protein...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...with some plasmids. You just need three plasmids to start making AAV:  the packaging plasmid which contains... transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ...of an AAV transfer plasmid. Parts shown in green are key elements of an AAV plasmid. Parts shown in grey... elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal ...transfer plasmids have a promoter, but this element isn’t required when the transfer plasmid harbors non-coding...Transfer Plasmid video!   Now you’ve got a basic understanding of some common AAV plasmid elements!...structural and packaging genes, the adenoviral helper plasmid which contains the proteins needed for the virus...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile... These two steps are (Figure 1): The initial plasmid integration step (the so-called “single crossover...versatile, synthetic MCS (designed with the help of the DNA tuner program) (Latynski and Valentovich 2014). We... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program for the construction of...

  11. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...giving you more choice in how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing our ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...countries enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment...next-generation sequencing, setting new standards for plasmid quality control. Through our partnership with the... viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged on demand...sequences and characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists...developers to unlock the potential of our extensive plasmid database — because we believe that innovation flourishes...

  12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding domain); and a UAS-driven luciferase reporter...systems. T-DDOGs can also be designed with other DNA-binding domains, including the commonly used LexA...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer...

  13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...introducing double stranded breaks (DSBs) into genomic DNA in many cell types and often results in biallelic...homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves...involves simultaneously introducing a homologous DNA repair template, such as a sin­gle-stranded oligodeoxynucleotide...at all, such as when you are editing a non-coding DNA region? To this end we developed a technology called...Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages Find Validated...

  14. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences...been most widely used to create specific edits in DNA, there has also been significant work on constructing... any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a transcriptional activation ... activators, similar tools were made using other DNA binding proteins such as zinc fingers (ZF) and TAL... several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References...they want to activate, we would recommend using a cDNA overexpression vector rather than going through ...and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis regulation and also...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...techniques are still used and efficiently transfer plasmid DNA efficiently into dividing immortalized cells ...virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing all the structural genes...crossover event between the vector plasmid and the packaging plasmid caused the Psi sequence to be placed...expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone your gene of...usually 293T cells, with your transfer plasmid and your packaging plasmids. For this, you can use the transfection...reach their limits when it comes to transferring DNA into primary cells or non-dividing cells. To target... (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert of interest as well as sequences...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...incorporate new information into DNA. This also requires an exogenous DNA template. HDR, however, is a very...so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018). For C...made it easier than ever both to engineer specific DNA edits and to perform functional screens to identify...efficacious to target the coding or non-coding strand of DNA. As a rule of thumb, we avoid target sites that code...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut, the error-prone nature ...although this is rarely done). When targeting a dsDNA break for HDR, the choice of target site is far ...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...flanking the DNA-binding cleft of Cas9, the fluorescent domains dimerize and prevent DNA binding in the... cell, the dCas9-CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while...unveiled a photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a... Additionally, high intensity exposure can cause DNA damage. Far-red light, however, can penetrate 5 mm...fluorescent reporter gene in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-...stimulation, the pdDronpa moieties move apart, allowing DNA binding and cleavage. This technique was applied ...deactivated the protein’s ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen...

  18. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...will be able to request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...sequencing to confirm the identity of the packaged DNA and serotype. Please note that Addgene cannot perform...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

  19. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...technology to engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect ...as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened...HyPer3 (2) which he will co-express together with a plasmid encoding glycine oxidase. The goal is to determine...excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to...

  20. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    .... pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid. It does not include...AraC and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC...team tested different linkers between VVD and the DNA-binding domain of AraC using a mCherry reporter downstream...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace mCherry...PBAD promoter. If you already have a pBAD33-based plasmid expressing your gene of interest, all you have ... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...
Showing: 241 - 260 of 383 results