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We narrowed to 473 results for: val

Showing: 241 - 260 of 473 results
  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...from Addgene!   Figure 1: Experimental validation for Anti-V5 [IPI-SV5-Pk1] in a western blot assay...efficiency of this dual-plasmid approach makes it a valuable resource for advancing our understanding of its...
  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... line across the peak will indicate the cut-off value for a positive signal (Figure 8). Note that the ...Huwait, E., Gari, M., & Al-Qahtani, M. (2018). Evaluation of in vitro chondrocytic differentiation: A stem...
  3. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...click chemistry reactions instead of biotin — a valuable development in and of itself. After adding one... reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D Atlas.  ...
  4. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...career advice, open doors for you, and teach you valuable lessons along the way. We think it’s so important...in lab to go to with questions and concerns is invaluable. With a little thought you can build meaningful...
  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas...0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid vectors for...
  6. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of the reasons ...https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector...
  7. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... been full of bad news and dreadful prognoses. Survival rates for mesothelioma are creeping upwards, and...greater impact on the life expectancy and overall survival of mesothelioma patients. However, no matter how...
  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http://wesharescience.com...audiences that may otherwise never learn from the valuable research we are doing. Browse the site, learn ...
  9. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...whacky cell lines”. This may certainly have been a valid concern for the discipline (or lack thereof) in ...colleague?”. The main difference is the QC and validation carried out by the commercial repository. Repositories...
  10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...notebook starts with defining your lab’s needs The values of ELNs show in the long run as they enable researchers...part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities such...
  11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your gRNA Additional ...Plasmids for Plants Read Our CRISPR Guide Pages Find Validated gRNAs for Your Next Experiment ...
  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells, an idea...approaches, and each of these gRNAs will need to be validated for high on-target binding and low off-target ...
  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   LexA/lexAop A second binary ...plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan...
  14. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...verification approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only...resources on the Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies...
  15. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...mice and a Huntington's disease model) will be valuable resources for future studies of the molecular ...of materials engineering as well as extensive validation of the cell and gene therapy, so please check...
  16. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...doi.org/10.1534/genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...
Showing: 241 - 260 of 473 results