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We narrowed to 456 results for: val

Showing: 241 - 260 of 456 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells, an idea...approaches, and each of these gRNAs will need to be validated for high on-target binding and low off-target ...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...species, so be sure to check the literature and validate your choice of AAV vector for your experimental...administration doses. Don't forget to submit your validation data after your experiment! Thanks to Addgenies...

  5. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...verification approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only...resources on the Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   LexA/lexAop A second binary ...plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...doi.org/10.1534/genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...screening or easily shuttle the optimized, expression validated GPCR sequences to any desired backbone.  Kroeze...26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi Arumugaswami lab at...convenient experimental use. The plasmids were validated by both Western blotting and immunohistochemistry...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...cells an AAV can infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, ...235 (2014): 331-348. PubMed PMID: 25107853. 12. Valdmanis, Paul, and Mark A. Kay. "Future of rAAV gene therapy...

  10. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Square. But I made the sacrifice in April because devaluing science has alarming implications for our public...better funding, and helping others understand the value of science is an ongoing and difficult job. I urge...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...propagate your plasmids in: DH5α, NEB Stable, or ccdB Survival.­ Whenever possible, we propagate plasmids in ...such as Gateway vectors, we recommend the ccdB Survival strain. Figure 10: Enter the growth and distribution...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...significant A to non-G conversion at target loci. The removal of inosine from DNA is likely infrequent, thus ... Browse CRISPR plasmids for base editing Find validated gRNAs ...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...strengthened during memory formation and memory retrieval. In neuronal tracing experiments, brain tissue...neuroanatomy and neural connectivity, providing valuable insights for neuronal communication. Viral vectors...

  14. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...provide researchers with a collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first...

  15. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...to know where to look and how to start. There is value, then, in having a guide that curates this information...

  18. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...blog Newly Updated AAV Data Hub Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody ...
Showing: 241 - 260 of 456 results