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...legal status of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits...setup, there are no explicit instructions on what to do when something fails. While we’re sure our techs ...have to individually keep track of any failures and do troubleshooting on their own. For a few samples this...everyone up at some point, decide early on what to do in a namespace collision. It may even be worth it...

...continue reading for more details. How much time do you spend reading scientific literature? Very often...’t save you the hard work of reading papers and doing experiments but we can definitely give you credit...envisions a world where the impact of teaching and doing science is rapid, concrete and open to innovation...

...stability Figure 1: So many choices! What to do? The main factors that can affect DNA stability...stability of your DNA, it will limit what you can do with these samples. This is because divalent cations...than water in these conditions.  The next time you do a plasmid prep, plan in detail before you drop that...

...researchers are communicating across disciplines or doing different tasks. But these challenges can be exaggerated... with each other in person. One possibility is to do a lab exchange, where researchers spent time in each...cohort, neither of which I would have been able to do from my home lab. Scientific conferences and workshops...

...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of...you’re seeking. Fortunately, there are many ways to do this involving positive selection, negative selection...cells that contain a specific gene survive. The rest do not grow. While there are many ways to design your...

...compelling, and I encourage you to hold one. Why do we need physical models of protein structure? Structural... you can only wonder what I am talking about. How do researchers use physical models of protein structure...and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We...human body?” … and probably most importantly… "how do we know this?” Over the past fifteen years, the CBM...

...Entrepreneur, Motivational Speaker Do you have a difficult supervisor, or do you have to work with difficult...problem and leave your emotions out of the discussion Do not assume that your supervisor is familiar with ...ideas, but he or she probably has reasons for it. Do not take criticism personally or get defensive. Look...

...primary antibody controls in your experiments. If you do find high levels of background staining by the secondary...binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your secondary antibody bind endogenous...common solutions to avoid background staining when doing species on species IHC. (A) One solution is to use...

...2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions The answer is, yes, it does...Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound to an...structure and isotype determination, remember that it can do so much more than that!    References and resources...

...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...they are directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame...