We narrowed to 783 results for: INA

...protein tag which can be added to either the N or C terminal of a protein and can inducibly facilitate the ...concentration can yield intermediate protein abundance. Finally, some degron tags can do more than just degrade... the gene of interest and the degron domain is terminal. Between the tag elements (protease and degron...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...protein-of-interest and the CRBN E3 ligase complex leading to ubiquitination and proteasomal degradation. Adapted from Nabet...interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in question, it ...complex members, an E3 ubiquitin ligase complex. Finally, when auxin is added in the form of auxin hormone...

...did not result in significant recombination. If you do see recombination, we recommend ensuring the cells...pooled libraries can lead to issues such as recombination and loss of plasmid diversity, so Addgene strives...strives to distribute aliquots of the original library whenever possible. In this blog, we’ll discuss ... lentiviral backbone might be more prone to recombination, while a library with a large backbone might...diversity of the library is maintained and reduce recombination during the amplification process. In liquid ...factor overexpression library) or is prone to recombination, we recommend amplification on solid media. ...may not have amplification protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all...

...researchers test many different combinations of promoters, terminators, solubility and purification tags...connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of DNA parts...EcoFlex a great option for those interested in recombinant protein purification and metabolic engineering...expression, the system includes a toolkit to express recombinant proteins within the mitochondrial matrix.   ...specialized system with parts including site-specific recombinases, regulatory elements, developmental regulators... examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions in...localization and transport studies in HEK cells, recombinant protein purification in bacterial cells, and ...

...validation data, or follow the references to find the original publication and ensure that it includes validation...-lot. The highest reproducibility comes from recombinant antibodies (rAbs), which are produced from plasmids...plasmid sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different...re-purchasing your primary antibody with the new reporter. Finally, each new conjugate will have to be tested to ensure...It is recommended for small to medium proteins. Finally, there are dry/electroblot transfer systems available...range, they cannot be read with equal accuracy. Finally, they degrade quickly and need to be read fairly...compatible with stripping and probing.   Imaging At the final step of your western, you’ll have two choices: x-ray...

...Cas13a: An RNA cleaving CRISPR nuclease Cas13a was originally identified in 2015 (Shmakov et al., 2015). They...enzymatically “active” state rather than reverting to an inactive state like Cas9 or Cpf1. As a result, Cas13a will...sequence of interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription...crRNA targeting the sequence of interest and an inactivated fluorescent RNA reporter. If the target sequence... whether the target sequence is present in the original pool of nucleotides.   What are the potential...regulating expression of specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity...-targets. They subsequently used catalytically inactive dLwaCas13a as a programmable RNA-binding protein...

...presence of that metabolite (Fig.2). Spinach eventually evolved into Spinach2, a more stable and brighter version...essentially mimicking GFP.  Though the aptamer, called Spinach, required longer exposure time than its protein...possibilities. For instance, further selection of Spinach with a target metabolite creates an aptamer that...but it still had limitations in vivo. Using a combination of SELEX and fluorescence-activated cell sorting...also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence...Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina and Jaffrey, Samie R. “Fluorescent RNA Aptamers ...Activity with GFP Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene...

...hinders experiments is mycoplasma contamination. Mycoplasma contamination has been shown to arise from a ...Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention... growth and behavior. Signs of a mycoplasma contamination can be subtle and may include reduced saturation..., labs should routinely test for mycoplasma contamination. There are several commercially available mycoplasma...PCR-based approaches to detect even low levels of contamination. For those labs that prefer a more economical....C., Drexler, H.G. “Detection of mycoplasma contaminations.” Methods in Molecular Biology 946 (2013). ...

...coli strains are not usually well suited for recombinant protein expression. Many challenges can arise...coli. We will review the potential pitfalls of recombinant protein expression and some of the most popular...or are enzymatically active. For this reason, recombinant proteins are typically expressed in E. coli engineered..., which reduces proteolysis of the expressed recombinant proteins. lon protease: Strains where this is...proteins. hsdSB (rB- mB-): These strains have an inactivated native restriction/methylation system. This means...plasmids utilize inducible promoters, which are 'inactive' until an inducer such as IPTG is added to the...to make protein.  The DE3 lysogen/T7 promoter combination is the most popular induction system. The DE3...

...AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban.... These preps drive expression of either Cre recombinase or a fluorescent marker in the various cell types...serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype AiP13778 - pAAV-...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13779 - pAAV-AiE0779m...minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13781 - pAAV-AiE0452h-minBG-iCre...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases Jonathan Ting New viral prep pAAV-CAG-GFP ...

...dietary supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate Professor... viruses. Now focusing on plant biology, she coordinates the Network for the Genetic Improvement of Cowpea... order to manage insect pests and to support sustainable agriculture. For this project the expression ...LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened for toxicity...oxidase. The goal is to determine potential water contaminants and to subsequently develop technologies to ...who applied, and to Seeding Labs who helped us coordinate and promote the awards. References 1. Martinez-Klimova...