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We narrowed to 320 results for: POR C

Showing: 131 - 140 of 320 results

  1. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...10.1126/science.1206593 Powers S, Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein....2020.107878 Sinnett SE, Hector RD, Gadalla KKE, Heindel C, Chen D, Zaric V, Bailey MES, Cobb SR, Gray SJ (2017... More recently, the Bird lab found that only a portion of the MECP2 protein is necessary to rescue the...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878...

  2. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ... by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a toadfish...calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all of the standard...said. “We know CREB is really important, but where is it really important and when? That is not known.”...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be used quickly...PubMed Central PMCID: PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of ...Axonal Transport It has been demonstrated that certain serotypes can undergo axoplasmic transport. These...of neurons (retrograde transport) or towards the synapse (anterograde transport), which is a possible ...retrograde and anterograde transport, but they also have similar transport characteristics (velocities...strong CNS tropism (4). If axonal transport is not desirable, transport can be reduced by injecting a smaller...and using AAVs have in common? The three most important items when looking for a house and choosing an...

  4. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F. (2019). Plant gene...0337-2  Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021). Precise plant genome editing using...Gao, Q., Liu, S., Zhang, X., Hu, W., Wang, Y., Han, C., Li, D., & Wang, X. (2025). Transgene- and tissue...these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...mEosFP’s chromophore requires a temperature below 30°C and this is not ideal for experiments in mammalian...can form it’s chromophore at a more comfortable 37°C (5). Dendra2 photoconverts from green to red fluorescence... pH stability, chromophore maturation rate at 37 °C, turnover rate, and the level of background fluorescence...: 1035-1040. PubMed PMID:18724362. 8.Stiel, Andre C., et al. "Generation of monomeric reversibly switchable...impossible to determine specific protein turnover or temporal expression using standard FP-chimeric proteins...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...localization, or leading to protein aggregation. Another important parameter to consider is the contrast ratio- a...

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) ...Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung... https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC... to cleave and activate a reporter. The detectable signal from this reporter can be measured and quantified...and a Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...variants, Cas13, and Cas12a, to develop simple, portable, and inexpensive platforms to reliably detect ...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...create solution C. And if a conflict resolver does their job well, not only will solution C be better than...many sources of conflict are substantive (“Person C told Person A one thing and inadvertently told Person... is customer support, but there are eight other teams who still retain a customer support element amongst... your technical skills, can open new career opportunities for you, if you can identify them in yourself...explaining my approach to conflict resolution, it’s important to recognize that there are different types of...or goal. Resolving these types of conflicts is important for the individuals involved, and also for the...responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day operations...

  8. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...constructs 69570 and 69571 together encode the N and C terminal segments respectively of a GFP dependent ...-N-CretrcintG Connie Cepko 69571 AAV1 pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW.... The advantages of optogenetics lie in the spatiotemporal control offered by the system that allows for... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...

  9. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...replicates such as multiple mice in each group and C, technical replicates or repeated measurements will...interactions between the sample and the secondary antibody. C, a label control treats the sample to the full protocol...PubMed Central PMCID: PMC5773976. Waters, Jennifer C. “Accuracy and Precision in Quantitative Fluorescence...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...

  10. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A...describing a technique that allowed for intentional incorporation of an unnatural amino acid in translation (Noren...natural translation machinery; these are often incorporated into the cells via plasmid. Third, a second ...containing the chosen codon that will result in the incorporation of the non-canonical amino acid at the site ...integrated into the cell’s genome, instead of incorporation via plasmid or media. Theoretically, if you ...will produce a protein with a site specifically incorporated ncAA, ready for you do to any number of experiments... They provide resources, knowledge, and most importantly, validated protocols (using readily available...
Showing: 131 - 140 of 320 results