We narrowed to 884 results for: ica

...regular part of basic biological research as well as clinical therapy. These biological tools are useful because...being engineered as biological tools, thought was given on how to keep the replication of virus and its ...the component that physically integrates into the host genome, the virus is replication incompetent, meaning...envelope plasmid), and the polymerase (Pol) for replication and Gag structural protein on yet a third plasmid...meaning it can’t replicate and continue the infection cycle once inside the host. While the virus can still... this safety feature prevents the virus from replicating and spreading in non-target cells, including ....    Viral cargo-specific safety   After replication competency, the second concern with virus safety...

...in PubMed and choose the appropriate publication. This publication will be listed at the top of the final... with publication details or permission to distribute, or Addgene finds your journal publication describing... "Published plasmids" or "Unpublished or pre-publication plasmids" and set a desired distribution status...quickly associate your plasmids with the original publication where you described them. Search for your article...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that...accepted. Addgene also encourages submitting pre-publication plasmids so they can be available when the paper...preprint submission, we are happy to update the publication information once the manuscript is published ...

...Finding an antibody that works for your application, specifically detects your protein, is species compatible...(stay tuned for our affinity purification tag blog!).   Applications of epitope tags   The...protein purification is not ideal with Myc, as low pH conditions are required during the purification, which...and have been widely used across species and applications. In this blog, we will review the most common...that are bound by antibodies for the purpose of purification or detection of the tagged protein. These peptides...not exist, or they don’t exist for a specific application, or they exist but are unreliable for a variety...almost certainly already been optimized for your application. Depending on the tag, directly imaging or tracking...

...of its biggest limitations is that sequence modifications can only be made at restriction enzyme cut sites...homologous recombination. It allows for direct modification of DNA within E. coli and is independent of ...engineering.  It can be used to make an assortment of modifications: insertion and deletion of selectable and non-selectable...however, only Beta is required when generating a modification with an ssDNA substrate.  Gam: Gam prevents ...insert site (Figure 2, top).  These primers are typically ~70 nucleotides long (20 nucleotides that anneal...regions flanking the target site).  The best applications for dsDNA inserts include: large insertions ...fragments, such as gene replacements and tags.  The typical frequency of recombinants is 1 positive clone out...

.... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...most inclusive databases of post-translational modifications, identifies a staggering ~250,000 phosphorylation...phosphorylation events on the activity of a given protein? Classical methods for studying protein phosphorylation A...steric and charge-based nature of the phosphoryl-modification; they are both much smaller, and carry only ...thus cannot reflect the true state of protein modification within the context of its appropriate regulation...plasmid. This includes 4 copies of a tRNA that specifically recognizes amber stop codons (TGA) to encode...effects on cell growth. The C321.ΔA strain was genomically recoded to eliminate all 321 amber stop codons...

...2012) scFv Antibody: Principles and Clinical Application. Clinical and Developmental Immunology 2012:1...therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed et al...scFvs are often genetically fused to other proteins, which has led to their application for basic and translational...cheaper to generate since it’s genetically encoded.   Translational applications of scFvs: Just like antibodies...blood clearance, which is useful for imaging applications; better tissue penetration which is useful for... for therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to... translational research.  Basic research applications of scFvs: The SunTag system uses an scFv to amplify...

...ASAP5, a faster and more responsive genetically-encoded voltage indicator (GEVI) than previous versions. ASAP5...responsivity in screening ASAP3 variants. B) Electrical and optical recordings of spontaneous activity in human...protein-coupled receptors (GPCRs) are critical components of many physiological pathways and represent the largest...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...Arabidopsis, suggesting the improvements may be broadly applicable to dicot species. In addition, they generated...

...Foundation, an organization working to advance biological engineering openly so as to benefit all people...Initiative. Endy's team made news in March with a publication in Science describing the development of “transcriptors...with the BioBricks Foundation to distribute the physical DNA samples, for when that's the preferred mode...parts must flow.' People must be able to use genetically encoded functions - the words in our language...operating system for engineering an entire cell. Typically when people use plasmids, they are trying to do...the entirety of a cell. That's a big long-term technical challenge. We have a strategy that organizes how...work, and it begins with the central dogma - replication of DNA, transcription of DNA to make RNA, translation...

... be run in duplicate or triplicate. The closeness of the data points generated by replicate values provides... the standard dilution series in duplicate or triplicate. Replicates allows you to calculate the curve...blot. Standard curves are critical for a number of popular scientific applications, and the quality of a ... deviation of each replicate measurement divided by the mean of all the replicates multiplied by 100. ...regularly, and mix samples very well.  Use duplicates or triplicates to increase the accuracy of your experiment... value. Though it varies from assay to assay, typically the experimental value should be within 15% of...correlation between the actual values and the predicate values and measures how well the data points fit...

...caudal NST. (A-C) Low-magnification images showing viral spread. (D-F)  High-magnification images immuno-stained...vector performance. Yet, most of the technical data needed to replicate and build on others’ results is available...Produce Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development...and community concerns AAV vectors are complex biological reagents; many factors, like storage buffers ... right AAV for your system or to successfully replicate published works. Of particular importance to these...improve our Addgene Data Hub, 76% of respondents indicated they wanted to see more negative data (Figure ...out of fear it points to flaws or gaps in their technical ability. But, especially for AAV data, that may...