We narrowed to 396 results for: organization Addgene
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TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...demonstrate the new system in a video filmed at Addgene: If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog: Flies 101: Catching flies in the lab References...
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New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be familiar...forming the cilia that help establish left-right organization of the embryo (Aljiboury et al., 2023). It’s...and cilia formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https...chromosomes during mitosis, support transport of organelles and vesicles, and more. Although several strategies...family proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing... Various Addgenies... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Services Resources on the Addgene blog Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications... to AAV Find AAV Calcium Biosensor Vectors at Addgene ...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...shipped 2,078 items to 764 scientists at 375 organizations in 21 different countries. It's safe to say ...publication by pasting the paper's title into the Addgene website search bar? We'll be celebrating all ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be.../10.1002/iub.165. Additional resources on the Addgene Blog CRISPR libraries Genome-wide screens with... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...based biosensors. Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....Additional Resources on the Addgene Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists share...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....information to Addgene Material Transfer Agreement (MTA) approval Send plasmids to Addgene Quality control... field, and contact Addgene. We may require that you send your samples to Addgene pre-transformed in this... to appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping... Additional Resources on the Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable...the spreadsheet to deposit@addgene.org with the following information: Addgene account username. In order...Suggest A Plasmid You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which...tech transfer team will communicate with your organization’s technology transfer office or other authorized...