We narrowed to 402 results for: LARGE;

1. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction enzymes needed Not...

2. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...

3. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ...diagnosis indicate, for Stage 3, that the cancer is large in size and may have spread while Stage 4 indicates...

4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...about the advantages of pooled CRISPR libraries for large-scale functional screens Read this blog post about...

5. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ... purification procedures. Even if you produce a large amount of protein in your expression host of choice...

6. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...

7. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ...Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced ~1,500...

8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...insertions and deletions (indels) most likely to have large phenotypic effects such as those that affected gene...

9. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...effectively a million dollar sales pitch. Given this large sum, one might expect that applicants would work...

10. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ... vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out...

11. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...from purified recombinant proteins at small and large scale.  This new technique involves selection in...

12. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral vectors, they...

13. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...software was originally designed to help us work in large formats, such as 96-well microplates, and thanks...realized the need to modernize our approach to handle larger volumes of data. In this two-part blog series, ...

14. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ... and structure. Their porous surface provides a large area for binding, but antibodies conjugated within...

15. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...

16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ...

17. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ... did, and state that it would help you manage a large number of files as part of the job. Structure of...

18. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...

19. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...variants use different RT enzymes and performed large edits with similar or better efficiency than PEmax...

20. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized...