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... questions about the ordering process, the Addgene Help Center is full of information about how to deposit...requires a quote in order to generate a PO, learn more about how to create a quote. How do I pay the invoice...Addgene Help Center If you have additional questions about our ordering process not addressed in this article...

... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....dominates DNA quantification discussions, but what about the other methods to quantify DNA? Here we cover...Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...

...ChIP-seq methods provide a wealth of information about how protein networks work in cells. A major bottleneck...two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA Technologies, USA). These...commercial suppliers without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted...first crRNA we designed for ~250 genes, we have had about 30% tagging success, although many of these genes... When not pipetting, he can be seen daydreaming about sunny days in the UK.   References 1. Bressan RB...

... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...to consider these cases separately when talking about the tools that are available. Let’s consider the...that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass into your protein and ...together The FRET donors and acceptors described above can be paired together to measure a wide range of...of your protein that can lead to novel insights about its biology and its behavior. See the table below...

...post, a study coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful...conversations within the team. We are not talking about gossip here – this means encouraging a culture of...Medical School researcher Connie Cepko to learn about her management style.   Seeking feedback frequently...information to make change whenever possible. Be public about changes influenced by feedback. If you don't do ...

...Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene...Addgene could not do what it does.  Melina talked about her grandfather’s humble beginnings as an operator...and add to this post. Interested in learning more about the last 10 years @Addgene Check out our impressive...stats from 10 Years of Plasmid Sharing Or read more about what happens Inside Addgene   ...

...the Addgene blog Learn more about Addgene's new search tool Read more about other features of Addgene's.... Instead of trying to place all of the content above the fold, we decluttered the page elements and allowed...comfortably tell our story, to get scientists excited about what Addgene has to offer, and to help scientists...

...not even get a solution to the problem you called about. Next, you try an email, given that this is the ...” next to an encircled question mark (see image above). This useful widget allows you to access our Help... or you just have a general question or concern about the answers we provide, within the widget you also...Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources at ...

...nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned above, however, Cas9 sometimes..., 2022), indicating that there is more to learn about this process!      Figure 3: Crystal structure...rest of Cas9 and is highly flexible; it can rotate about 140 degrees between inactive and active conformations...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

...approached me and asked about my current projects and personal interests. We chatted about social media analytics...while eating lunch. There was something so special about her pure interest and curiosity to understand my...hours! As my time here comes to an end, my worries about making connections with coworkers seem, in retrospect...

...We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner...partner seqWell and going into detail about how we use next generation sequencing results to perform quality...control on deposited plasmids. We’ve also talked about how our new Snapgene generated maps provide improved...open reading frames, which is often our first clue about where the gene of interest might be located. By ...

...blog Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling ...the same genetic makeup, overcomes all of these labor-intensive and invasive techniques to reliably label...

... for the Addgene blog, I was focused on writing about new scientific techniques and cool plasmids. Creating... graphics were usually the last thing I thought about when writing posts. Since then I’ve realized my ...know of a tool or resource not mentioned? Tell us about it in the comments section!  Additional resources...scientists Find science career blog posts Learn about other lab software ...

...attended the Go green! Innovative practices for laboratory waste symposium, sponsored by New England Biolabs...Biolabs and Labconscious. I was eager to learn about practices I could bring both to Addgene, and share...offsets the cost of regular waste services. Learn about how to start a glove recycling program at your institute...lab tips for your research Read another blog post about conservation in the lab Resources on Addgene.org...

...sensors generally follow the same pattern described above - a sensing domain induces changes in a fluorescent...expressing a biosensor in eukaryotic cells, think about the best place for the biosensor to be expressed...MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent...Blog Check out the Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome...

...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

...scientists, including Nobel Laureates, who speak about their work and current, trending topics in their... The society facilitates opportunities to learn about Synthetic Biology and exciting new technologies ...opportunities for society members as well as arranging collaborations and conferences for their students. Another...interests. Scientific societies like those mentioned above provide strong foundations upon which young scientists...

...research question that people (especially you!) care about. Have you critically evaluated your plan? Have... to complete and has multiple benchmarks. Think about what your personal goals are and set milestones .... Take the time to generate new ideas and think about them critically before diving in. Research projects...Medical School) for taking the time to speak with us about adding new scientists to their labs. Read All the...