We narrowed to 820 results for: APP

...as #happydepositors who stopped by to say hello. Needless to say we all got very good at applying temporary...six continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware...

... though not all, of these defenses. For some applications of genome engineering - such as certain forms...and improved SB transposase, SB100X, that is approximately 100-fold more efficient than the first-generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

...team. It is pretty clear that if people are mostly happy at work, they will work harder and contribute more...contribute to job satisfaction: A feeling of being appreciated and valued This often stems from being included... Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental...

... supplies every 10 years or so. Actually, I was happy to hear that I am not the only Addgenie who has ...I started looking for smaller and smaller gel apparatus until I settled on using glass slides. With just...this one only cost a few dollars.  I used this apparatus for 15 years before I hung up my pipets.   ...

...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

...different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049...113049 offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h...

...chatting makes it pretty easy to make 30 minute appointments with people all over the world. Think about ...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get...

...Science magazine. That one I just could not resist applying for. Not that I knew anything about being an editor...constellation of interests and skills than what fits for applied research. I focus in general terms on a couple ...Review and Scientific Publication, where diverse approaches to peer review were discussed. Science communication...

...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...lack of site selectivity typically diminishes the apparent affinity for the ligand, especially if the modified...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which...

...artefacts in your experiments. Read this post from Erik Snapp, Associate Professor at Albert Einstein College ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action... to your DNA sequence of interest. Potential applications of the system include activation and repression...

...form has 5 general question types and it takes approximately 15 minutes or less if you have all of your data...under copyright, you will need to obtain written approval from the copyright owner and will have the opportunity...viewing the data you have any feedback at all, we’d appreciate you sharing it through this form. In honor of...

...on the marketing or business background of the applicant. However, although this position did look for ...experience or a marketing interest that would be able to apply themselves immediately to the position. In case ...understand enough to relate it to the position I was applying for. After all I was only going to be interviewed...

...your meetings productive: Discuss logistics if appropriate:  How often will you meet? How does the Mentor...the process of working on them is where growth happens. Mentors bring more than their knowledge and teaching... get a bunch of text messages asking me, “What happened? Where are you?” My gym posse holds me accountable...

...bioanthropology. Studying human health science made me happy, but it wasn’t long before I started becoming disillusioned...drives better after a few drinks. A family member approached me for advice on her baby’s health but adamantly...abstract where the real medical conditions had been swapped with made up illnesses. They were then asked to...

...origins of the system and explore advances that have happened since our original 2018 blog post. The origins... second generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved...about other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein...

...perform the experiments to demonstrate that this approach was useful in humans. Then, one of them heard ...has been the realization that there is a great untapped potential to make science more efficient, open...activity is non-profit and pro bono. If this sounds appealing, join us and help make science a better place....

...actually making it happen would increase dramatically. I’m not sure what the best approach to doing that will... communication about experimental methods and approaches. It’s very easy to think that the methods section...

... the self-assembling capability of sfCherry approximately 20 fold by directed evolution. They then optimized...concept that the designed used in FlipGFP can be applied to other fluorescent proteins to create an array...and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage...

...virtually) will make the relationship stronger.  If appropriate, invite them to speak for your organization, ...doesn’t work out, it’s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who...with peers and people around you naturally. This approach is much more likely to pay off in the long run...

... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

...used Parkinson’s experimental model. In fact, approximately 25% of yeast genes have a human counterpart,...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms transcribed...single TF was delivered to each cell, future applications could deliver multiple TFs to each cell, allowing...and/or TF dysregulation. A targeted TF screening approach can not only lead to a better understanding of...

...(Schrallhammer et al 2012). This made them an appealing target for bioengineering, but it wasn’t clear...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...

...flank the insert. You will need to know both the approximate size of the vector backbone as well as the predicted...you have loaded in each well will allow you to approximate the DNA mass of comparably intense samples of...the gel when the bromophenol blue dye line is approximately 75-80% of the way down the gel will ensure you...

...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...dream lab While none of us will feel completely happy at work all of the time, the goal is to spend as...the difference. Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments...

...guidance and structure. I quickly learned that my approach needed to adapt based on individual needs. For... and disengagement. By tailoring my leadership approach to each person, I was able to create a more productive...tension between two team members with differing approaches—one detail-focused and the other big-picture ...

...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...intermediate won’t just have nicks: it may have gaps or overlapping regions of single-stranded DNA. But as long as...of multicomponent assembly, as fragments with overlapping sequence homology can be assembled in a unique...

...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some... after less than a minute of exposure to the appropriate light. The photoactivatable counterpart to this...protocols to get your CRISPR experiment moving. Happy editing! References and Resources References Hendel...

... and remove a pathogen. Different isotypes also appear at different stages of an immune response. There... isotype of an antibody so you can select an appropriate isotype control. An isotype control has the same... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...