We narrowed to 435 results for: Trac

1. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people to do ...

3. An Inside Look into Addgene's Dev Team

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   Blog Post

   Published

   Jan. 23, 2020, 2:45 p.m.

   ...Addgenies, development includes everything from tracking plasmids from the moment a deposit kit is sent...

4. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ... by your presentation, then you’re on the right track. Furthermore, practice getting back to your story...

5. Plasmids 101: Codon usage bias

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   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...properly. For example Pechmann and Frydman found that tracts of non-optimal codons are associated with specific...

6. Transferable Skills Guide: External Collaborations

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   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral...

7. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ...

8. How to Write a Scientific Review Article

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   Blog Post

   Published

   Feb. 16, 2017, 3:30 p.m.

   ...This is normal and it means you’re on the right track!  Get feedback After you’re done editing, send it...

9. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ...Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance...

10. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ...today, but it doesn’t have to be some looming, abstract, convoluted scientific concept. It is imperative...

11. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How ...

12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    Published

    Oct. 13, 2016, 2:30 p.m.

    ...roles. Healthcare consulting is a demanding but attractive choice for anyone who wants a fulfilling career...

13. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...examples I’m personally interested in include: intracranial self-stimulation (ICSS) and place preference...

14. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ..., we will take a step beyond discussion in the abstract and follow an example of a plasmid-based experiment...

15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...expression of the transgene. This makes IDLVs an attractive choice for certain types of clinical or basic...

16. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...The source of those conflicts can frequently be traced back to one of a few trouble spots:  Communication...

17. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...focus on you and your ideas. Slides will be     distracting. What is the big question? This should be...

18. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...

19. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ... promise! FSC and SSC: scattering To digitally extract your cells based on their size, the cytometer provides...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...labeled ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the ...

21. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ...else have a good answer for this?” This doesn’t detract from the speaker, but actually makes for a more...

22. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...efficiency of the dephosphorylation reaction can be tracked through the comparison of phospho:non-phospho species...

23. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...problem of in vivo CRISPR delivery become more tractable in the next few years, as it would open up many...

24. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ... cloning) and SLiCE (Seamless Ligation Cloning Extract) to name two more. Likewise, there are several ...

25. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...14.3% of PhDs in the life sciences end up in tenure track position 5 years after receiving their PhDs, still...

26. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    Published

    Oct. 2, 2018, 12:56 p.m.

    ... also be a sign that the lab head has trouble attracting scientists. To get a better idea of why the lab...

27. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ... characterised antibodies against all human extracellular proteins with community-based validation. They...

28. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...

29. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...

30. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ... am not sure that I want to pursue the academic track.” How does an International Mentorship Program work...