We narrowed to 814 results for: Ran;

...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our...

...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for your... reactions are performed, the next step is to transform competent E. coli cells and select the positive...Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley...

...pharmaceutical companies, biotech start-ups, health insurance companies, and governments on the most effective...physicians and patients, shaping discussions with insurance companies about the pricing and coverage of drugs...therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how our clients’ ...When I was first considering preparing for and transitioning to a career in healthcare consulting, I found...

...dDetermined as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

...looking, I found an inexpensive HDMI transmitter that could transmit a signal between a wall. There was ... things first was getting that wireless HDMI transmitter/receiver, to beam the camera’s image to my remote...then set up a monitor connected to the wireless transmitter and an iPad in the office next door (connected...

...types of ELNs are available on the market today, ranging from general note taking tools, to more scientific....e. main priorities of different ELN features, ranging from whether respondents saw them as not important...requires time and knowledge. In a recent article, we translated our knowledge of electronic lab notebooks into...information. If an employee leaves the institution, the transition is smooth as all data is organized within an ...

... avidin, proximity labelling can capture weak/transient protein interactions as well as be used for both...proximity labeling by promiscuous biotin ligases (Branon et al., 2018). BioID and BioID2 can take hours ...2019). This technique relies on an S. auerus transpeptidase sortase A variant (mgSrtA), which is capable...motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity labeling ...

...While this may effectively serve as a transfection or transduction control, a different gRNA would have..., you’ll want to select a Cas enzyme with low tolerance for mismatches within the gRNA sequence. ...near each other, this will result in a DNA double strand break (DSB), the event required for frameshift ...

...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...don’t tell us everything there is to know is a strange and exciting revelation. Science in any field can...investigator (PI) Runs the lab Writes a lot of grants Varying degrees of engagement with what happens...1-2 years) Often a gap-year position taken to transition to the next career stage (ex: grad school, med...

...levels to generate anything from individual transcription units (TUs) used to express your favorite gene-of-interest...service (Lee et al., 2015). This kit offers a broad range of parts, including copy number machinery, chromosomal...vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized...used successfully for protein localization and transport studies in HEK cells, recombinant protein purification...

...Although white light is entering your hand, only orange/red light can be seen exiting through the tissue...detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment,...Reversible Saturable Optical Linear Fluorescence Transitions (RESOLFT/STED) also excel on these types of samples...when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation and...

... modified cells are transferred into an animal and the behavior of the transferred cells is followed. ...generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target...pathway with many receptors, signaling molecules, transcription factors, and ligands. The presenter then showed...

...Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University of Agriculture...-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects... transgenic crops...

...choices), we'll be in touch with you soon with some brand-new Addgene swag!   Last month, we introduced you...out to you and received a plethora of options, ranging from Affie to Yncredibody. After an agonizing week...their new friend…  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed ...

...Novophage's successful transformation into Sample 6 and Dr. Koeris' own transition from graduate student...37-36:53 : Was It difficult for Dr. Koeris to transition from developing therapeutics to developing a ...

...characterized by single, linear, negative-sense, single-stranded RNA genomes. The virus does all of its damage ... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...the current Ebola crisis, Science and Science Translational Medicine have made their complete collection...

...ample resources and a positive perspective on the transition to a post-academic career. This training was ... inadequate job of supporting science trainee transition into diverse, successful careers.  We’re not ...components of predoctoral and postdoctoral training. A grant from the National Institute of General Medical Sciences...

... strategy to enhance HDR repair using a single-stranded oligodeoxynucleotide HDR template covalently attached...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

... °C but preferentially nicks the non-target DNA strand. The lab then found gain-of-function mutations ...mutations that allows it to be repurposed for double strand breaks at target sites. Read the paper in Nature... of stable genomic integration and is easily transferred via conjugation.   Read the paper in Nature...

...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental... but with the right incentives (for example, by grant agencies), we can shift toward a more sustainable...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

...connection that many people honor. Talking with strangers is usually challenging but does get easier with...you might spend a whole day having to talk with strangers. Be bold in asking all kinds of people to connect...to share their stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just...

... LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic... a lentiviral vector containing a gRNA. These transduced stem cells can be implanted in irradiated recipients...four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME Knocks out...

...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar....lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

...life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

...data, more discussion, and more functionality. Transparency, both in what works and in what doesn’t, and...Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza, E., Croteau, J., Thakur, N., Jonigk, D., Davis...00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...and gRNAs whose expression varies depending on transgene design and delivery. They generated hPSC lines...

...historians have the most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines...Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains ...network of life (green), physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting...

... Today, that changes. Today, Addgene launches a brand new Europe office located in London and operated...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology...

... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (...

...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...

... have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While... Mouse studies, however, require a permanent intracranial implant to be surgically installed to allow ...receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...

...between my lab in Pennsylvania and a team in Paris, France. While efforts like these accelerate the progress...everyone can access data and drafts of manuscripts and grant applications in real-time so they can provide input...International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances permit...

...of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends... Portugues, Ruben, et al. "Optogenetics in a transparent animal: circuit function in the larval zebrafish...

...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than...

... research project establishes your professional brand. It offers colleagues insight into your interests...understand where the field is today? Have you talked to veterans in the lab or the field? What are possible pitfalls...how can you mitigate them? If you were to write a grant for this project, what would be your critical experiments...

... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...

...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

...sequences required for viral replication and transcription as well as reporter genes. To learn how you ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

..., K. phaffii is exceptionally good for post-translational modification of recombinant protein products...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination...