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  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

  3. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression...

  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

  6. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

  13. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. Creating a modular... the SEC-based system is both simple to use and highly efficient for C. elegans genome modification. ...

  14. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

  19. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...focus on discussion and brainstorming Though, we might be forced to work from home and/or be in the lab...experience more interactive. The button is on the right of the Share Screen control panel on the top of ...

  20. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  21. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...

  22. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  23. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...

  24. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

  25. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...

  26. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

  28. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

  29. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

  30. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...you that Twitter is a tool worth using, but you might be wondering how to get started. This blog post ...video from Science Media Savvy At this point, you might be be thinking social media could be useful, but...

  31. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...however, they are different viruses and thus use slightly different isoforms of these packaging components...research to reduce promoter interference and/or drive high expression of a transgene from an internal promoter...

  32. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...and their functions are more intuitive than you might think. So, if you’ve ever wondered what goes into...polymerase II promoter and terminator regions and are slightly different in sequence. Both LTRs do share common...

  33. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor of a ...on changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure...

  34. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ... and Andrew Hempstead. What do smell, taste and sight have in common, besides being one of the five senses...Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors are activated...

  36. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...mentoring group Find 5-8 colleagues that you think might be interested and willing to commit to the group...same place, a 1-2 hour lunch on a regular schedule might work. If not, perhaps a 3 hour breakfast once a ...

  37. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

  38. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

  39. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

  40. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

  41. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

  42. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ... often post a preprint at the same time as, or slightly before, submitting the manuscript to a journal...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

  45. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...are a multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms ...AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population. These pre-existing...

  46. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...1948 as a variation of the more commonly observed light emission by fluorescence. The widespread use of ...distances that are too miniscule for conventional light microscopy. Don't FRET - Use these FRET resources...

  47. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose it can be. Let... Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with NEB’s DAD Interview...

  48. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated to the...interest. The optical density (OD) - the measure of light absorbed by a solution - of these wells is plotted...

  49. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...for these RNA sequences (3 nM), however, was quite high and above what would be expected in patient samples...daunting. It took a true team effort and many late nights, not unlike most research, but the urgent need ...

  50. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw cycles and should be stored...resistance marker expression following transfection, higher-level expression after approximately 72 hours is...
Showing: 651 - 700 of 831 results