We narrowed to 868 results for: REN

...click the "Save" button and jump around to the different pages by clicking on the appropriate boxes at ...at the bottom of the page before moving onto a different page so that any data you enter is not lost.   ...to the backbone, and the vector type. Pro Tip! Reference the Addgene ID in the "Backbone Manufacturer" ...

... this new technology as helpful as we have!  References and Resources Additional resources on the Addgene...Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...

...JEI. Chapter 3 - JEI target audience and their current readership. Encouraging students to do science ...biology protocols Check out our molecular biology reference pages ...

... the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists. These programs ...Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks...

...Top7 receives the Nobel Prize. Created with BioRender.com. Top7 gets its name from “topology of protein...David Baker, Demis Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani...

...research. Listen to learn more about Dr. Kwapata’s current work and thoughts on the need for more scientific...biotech research in Malawi 11:41-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44...

...the calculator in the comments section below. References 1. Choi, Vivian W., et al. "Production of Recombinant...Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed...

...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways... The AAV Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV...

...activated T cells, precluding analyses of T cell differentiation beginning with naïve T cells. The Framework...improving CD8+ T cell responses to cancer.   References and resources Milling, L. E., Markson, S. C.,...

... Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening in...screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing a ...

...available when they are validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease... system, see Yamamoto and Sakuma’s protocol. Reference: Multiplex genome engineering in human cells using...

...letter abbreviation of the mutated amino acid currently at that position. In contrast, unmutated or Wild...  Matthew Ferenc...

...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...

...detailing Nobel Prize-winning research typically weren’t among the most cited. And, as scientific discourse...made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing what they call “...

...virus works its way into immune cells in ways that aren’t fully understood. Much of the action depends on...relevant scientific literature.  -In light of the current Ebola crisis, Science and Science Translational...

... buyer to a seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does...you listed during checkout. This invoice will reference your Addgene order number and PO number that you...

...talent in science related careers. Failure to strengthen the talent pipeline sets the world up for reduced...training programs. I believe in the academic apprenticeship system as a training ground for scientists,...

... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

...hisashii, BhCas12b that is functional at 37 °C but preferentially nicks the non-target DNA strand. The lab then...approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these anti-Cas12a proteins...

...Research currently under way in the Kotton Lab. One of Darrell's favorite projects - differentiating surfactant...

...or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast....org or +1 617-225-9000, or check out our MTA reference pages. So go ahead and use Addgene plasmids ...

... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...

...find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende...

...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes...

...strives to match up scientists across fields with different technical knowledge that could help fight COVID... both clinical care and public health for this current COVID-19 pandemic, and outbreaks that have yet ...

...controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete...

...never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because of this.... And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ...

...the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce...enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases for ...

...Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014...system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity...

...includes an antibiotic resistance cassette and you aren’t introducing an additional antibiotic resistance...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ...

...meiosis in biology. Lots of it is researching the different aspects of the subject you're writing and then...developing new online courses about unique topics that aren’t already offered by eScience Labs.  “We work with...

... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

...costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...

...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...

...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...

...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...

...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...

...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ...

...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...

...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...

...of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad Institute...Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University  University...

... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody...

...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...

...studying animals than plants, and biology teachers’ preference for using animal systems to demonstrate biological... a nominal fee for university professors, are currently available through TAIR with a new ABRC database...

...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...