We narrowed to 745 results for: TRI
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TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....about a hundred billion neurons and six hundred trillion connections (synapses.) In this post we’ll describe...
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REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research. With many changes... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog PostPublishedOct. 21, 2025, 1:15 p.m....different functionalities, such as fluorescence or triggering new cellular behavior like degradation or electrophile... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m.... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....relevant viruses for your system. This post was contributed by guest blogger Didem Goz Ayturk with edits ... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... could present something coherent or to quickly string together all the words that I could remember related... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary amine... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m.... to genome instability. Thus, it’s important to strike a balance between productive levels of R-loops ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....at Novartis. The ClonTracer library has been distributed all over the world and was recently used in a... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....This post was contributed by Johnna Roose. This post was originally published on Johnna's New Under The... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....Develop a research plan Most grant applications restrict the space available to describe the proposed work... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S.... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....et al. 2014). Simple knock-out experiments are trickier when it comes to long non-coding RNAs (lncRNA)... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....Bacteriocin plasmids: microbial offense One way to triumph over your neighbors is to eliminate the competition... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....throughout cells. VPR Description VPR fuses a tripartite complex with dCas9 to activate transcription.... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....guide-independent off-target deamination, an important attribute for therapeutic application. ABE8s demonstrate... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....Delivering dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the degradation... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....options, check out the 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or the MoBiFC... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....different and potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m.... culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The MLV Gag protein... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....Caenorhabditis Genetics Center (CGC) which maintains and distributes the strains all over the world. When I was at... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....development of therapies when expression must be restricted to specific circuits. Methods for targeting expression... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....This post was contributed by guest blogger, David T. Riglar Advocating for Science Symposium and Workshop... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....Through a partnership with genOway, we are able to distribute materials containing FLEx technology. Read the... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....The follow post was contributed by guest blogger Juliet Moncaster Leadership skills are amongst the professional... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m....This post was contributed by Dora Farkas of www.FinishYourThesis.com and is one of many posts in our ... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....stories, riffs or opinions that each panelist can contribute. The moderator should identify the funny, provocative... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m.... This mechanism is important for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....it requires intubation of the mice, which can be tricky given their small size. This technique is most ... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....editing (Maggio et al., 2014). Gonçalves said they attribute this phenomenon to the presence of terminal proteins... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....crosslinking captures chromatin interactions, a restriction enzyme digest cuts DNA into smaller pieces, and... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....ddPCR for AAV titration and find helpful tips and tricks in our blog post “Droplet Digital PCR for AAV Quantification... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m.... catch all of these potential mishaps before distributing the vectors? Viral genome sequencing Thanks ... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m.... now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....kinda. In some cases, antibody providers are distributing monoclonal antibodies derived from the same ... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....it did a few years ago, but I hope the tips and tricks above can help you navigate it in confidence. ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... important pathways. We invite feedback and contributions from all members of the research community as... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....This post was contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....This post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute...