We narrowed to 746 results for: TRI

...not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...

...selection can take the form of varied temperature, nutrient availability, drugs, and much more.   Workflow...

...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...

...epitope. Addgene: Do you have any additional tips, tricks, or words of advice for people who may want to ...

...of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery present in almost...

...was a little like tapping in the dark - a lot of trial and error.” The structural details revealed two ...

...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes...

...different functionalities, such as fluorescence or triggering new cellular behavior like degradation or electrophile...

... with viral RNAs. RNA viruses, like Zika, often trick host proteins into binding their untranslated regions...

...This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize...

...relevant viruses for your system. This post was contributed by guest blogger Didem Goz Ayturk with edits ...

... could present something coherent or to quickly string together all the words that I could remember related...

... to genome instability. Thus, it’s important to strike a balance between productive levels of R-loops ...

...antibodies in its bloodstream, the antibodies are retrieved first by drawing blood from the immunized animal...

...package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently...

...mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary amine...

...at Novartis. The ClonTracer library has been distributed all over the world and was recently used in a...

...This post was contributed by Johnna Roose. This post was originally published on Johnna's New Under The...

...Develop a research plan Most grant applications restrict the space available to describe the proposed work...

...doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

...et al. 2014).  Simple knock-out experiments are trickier when it comes to long non-coding RNAs (lncRNA)...

...Bacteriocin plasmids: microbial offense One way to triumph over your neighbors is to eliminate the competition...

...Delivering dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the degradation...

...from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple...

...development of therapies when expression must be restricted to specific circuits. Methods for targeting expression...

...throughout cells. VPR Description VPR fuses a tripartite complex with dCas9 to activate transcription....

...guide-independent off-target deamination, an important attribute for therapeutic application. ABE8s demonstrate...

...options, check out the 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or the MoBiFC...

... culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The MLV Gag protein...

...Caenorhabditis Genetics Center (CGC) which maintains and distributes the strains all over the world. When I was at...

...different and potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes...

...This post was contributed by guest blogger, David T. Riglar Advocating for Science Symposium and Workshop...

...Through a partnership with genOway, we are able to distribute materials containing FLEx technology. Read the...

...This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience...

...The follow post was contributed by guest blogger Juliet Moncaster Leadership skills are amongst the professional...

...This post was contributed by Dora Farkas of www.FinishYourThesis.com and is one of many posts in our ...

...stories, riffs or opinions that each panelist can contribute. The moderator should identify the funny, provocative...

... This mechanism is important for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s...

...it requires intubation of the mice, which can be tricky given their small size. This technique is most ...

...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...

... catch all of these potential mishaps before distributing the vectors? Viral genome sequencing Thanks ...

... now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of...

...kinda. In some cases, antibody providers are distributing monoclonal antibodies derived from the same ...

...it did a few years ago, but I hope the tips and tricks above can help you navigate it in confidence.   ...

...crosslinking captures chromatin interactions, a restriction enzyme digest cuts DNA into smaller pieces, and...

...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful...

...ddPCR for AAV titration and find helpful tips and tricks in our blog post “Droplet Digital PCR for AAV Quantification...

...editing (Maggio et al., 2014). Gonçalves said they attribute this phenomenon to the presence of terminal proteins...

... important pathways. We invite feedback and contributions from all members of the research community as...

...This post was contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana...