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  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...development of therapies when expression must be restricted to specific circuits. Methods for targeting expression...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...Through a partnership with genOway, we are able to distribute materials containing FLEx technology. Read the...

  15. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple...

  21. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...stories, riffs or opinions that each panelist can contribute. The moderator should identify the funny, provocative...

  25. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...kinda. In some cases, antibody providers are distributing monoclonal antibodies derived from the same ...

  26. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... This mechanism is important for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s...

  27. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...it requires intubation of the mice, which can be tricky given their small size. This technique is most ...

  32. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...

  33. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...it did a few years ago, but I hope the tips and tricks above can help you navigate it in confidence.   ...

  37. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...these systems in your research? Cary Valley contributed to updating this article. References Cox, David...

  39. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...viral genome (Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must ...

  42. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...with the desired fluorophores. In figure 4B below, triple colocalization is possible even when staining cells...

  43. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving CRISPR...

  46. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...

  48. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures...
Showing: 651 - 700 of 728 results