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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE;

Showing: 261 - 280 of 610 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...activated patterning of dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...GAG), and are (typically) tethered to the cell surface by a GPI-anchor. Image courtesy of IPI.   ...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...GABA neuron, for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...you want to know if they co-localize at the same place in the cell? Or you know that the presence of several...your microscope. Abcam's sample protocol is a good place to get started.  One important consideration when...the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split Channels...

  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output...we used to drive recombinase expression may be replaced by sensors that correspond to the desired inputs...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with a human gene. Advantages...comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater...

  7. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...restriction enzymes that allow your insert to be placed into the backbone in the proper orientation. For... restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper orientation downstream of... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...

  8. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ... characterised antibodies against all human extracellular proteins with community-based validation. They...experiments on similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000...their experimental designs. There are many more facets to reproducibility issues in the biological sciences...

  9. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...of the world, professional internationalization faces several obstacles; for instance there are few resources...career are common problems that many others have faced yet, still, this challenge did not hinder them from...evaluation of the pilot program IMP 2014/2015, took place on July 27th, 2015 at a dedicated international ...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect on your personal... next four years. But college, and science, are places for learning and growth! As you learn more about....org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 . https://doi....

  11. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming a vector that ... Alternatively, your gene and the marker can be placed under the control of two different promoters. Both... Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer vector...

  12. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...teamwork was what drew me to the school in the first place. The famous discoveries and experiments I’d been...at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that nickname...sometimes even necessary, to build a team in the first place? Let’s think about some of the advantages of team-based...

  13. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...Overview of using Lambda Red recombineering system to replace a gene of interest with an antibiotic resistance...as non-selectable DNA fragments, such as gene replacements and tags.  The typical frequency of recombinants....e. make changes to the third base pair of the adjacent 4-5 codons that alter the nucleotide sequence ...

  14. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...government, evidence to inform policies, a protected place to work, funding for science, and numerous other...every political persuasion) must be humble in the face of evidence. Stephanie Hays is moving from engineering...Harvard University to studying an evolutionary arms race at University of California, Berkeley. She is a ...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal ...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use..., 2019). LY6A is functionally expressed on the surface of brain endothelial cells in C57BL/6J mice, but...

  16. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

  17. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Are you following us on Twitter or Instagram (or Facebook or LinkedIn) but want to hear and see even more...behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be...

  18. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem lab office looking...

  19. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...to younger graduate students   Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and he tweets...

  20. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ... tag a protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple...high-throughput applications. Perhaps IT5 and IT6 will even replace our older epitope tags - only time will tell! ...
Showing: 261 - 280 of 610 results