We narrowed to 293 results for: ADA

1. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   Published

   Nov. 3, 2016, 2:30 p.m.

   ...published in scientific journals flies under the radar and gains little notice. A 2009 research study found...

2. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...nucleotide-based ligand binders would prove to be highly adaptable tools. The key to aptamers’ flexibility is their...

3. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

4. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...(2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell...

5. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

6. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...

7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...

8. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   .... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...

9. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...

11. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...

13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...

14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...

15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...Widrick, J. J., Messemer, K. A., Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz...

16. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    Published

    June 27, 2023, 1:15 p.m.

    ...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...

17. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated...

18. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license...

19. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...

20. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    .... Jonkers, M. van Lohuizen, A. Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for ...