We narrowed to 820 results for: App;

...exist in the real world and corrective approaches and appropriate controls are required to make this method... this technique has revolutionised the way we apprehend molecular complexes and is still a very useful...experiment. You can then choose the most efficient approach for your own system. The simplest and the most...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...They generated variants optimized for different applications (v82 has higher affinity for glutamate but slower... in the fluorescent properties, pYtags are an appealing tool for monitoring RTK signaling.  Figure 4:...lower binding affinities to broaden the range of applications. Image reproduced from Torres Cabán et al. 2022...screening platform developed here is also a promising approach for developing sensors for other targets. Figure...

...sell them. Types of Conflict Before explaining my approach to conflict resolution, it’s important to recognize...day-to-day operations and keeping our customers happy, but inevitably there will be gray areas that either...you can help everyone walk away from a conflict happy, feeling they each got enough of what they wanted...Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based on various levels...yourself gravitating most frequently towards these approaches, you may need to put more conscious effort into...

...decades. Widely used across various research applications, they have become indispensable tools for manipulating...-lasting changes in cells. However, for some applications, integration might be considered a double-edged... certain types of clinical or basic research applications. Considerations for IDLV use If you’re reading...Integrase-defective lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org...lentiviral vector: prospects for safe clinical applications. PeerJ, 10, e13704. https://doi.org/10.7717/...

...one of two ways - each junction resolution could happen on the crossing strand (horizontally at the purple...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)... PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS...

...molecular biology applications for biotin and avidin. A short list of some other common applications and links...downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications including... feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application of avidin-biotin technology to improve cell adhesion...

... talks and how to make them serve you well. The happy byproduct might just be a career opportunity. The...non-academic settings. Many of these guidelines will apply to any type of science presentation, but, for more...and venue before tailoring each presentation appropriately.  Find out who will be listening.  Are they ...goal is to get the listeners to be thinking “What happens next?”  Describe how you pushed to a solution or... use no notes and can concentrate on delivery, rapport with the audience, and their own excitement about...

...ways to clone DNA fragments which have provided appealing alternatives to restriction enzyme cloning. These... amplification with primers that contain the appropriate homologous sequences. NEB recommends an overlap...sequences remaining in the final product. The long overlapping region between fragments also better ensures ... of the fragments as compared to the smaller overlapping sequences left with a restriction digest. A study...cloning kits available which are all based on long-overlapping regions, have no requirements for restriction...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place....org with any thoughts, questions, or feedback. Happy reading! ...

...it got “dirty”, with multiple sequence traces overlapping each other! When the count was done, 75% of the...but not least, when using CRISPR, remember to appreciate the fact that it was first discovered in an obscure... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-...

...from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More #agarart from our lab @SILS_UvA...display @Micropia! #SciArt #MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega...recycled lab materials into holiday decorations. We appreciated the creative uses of old posters, pipette tip...to New York City. Check out their video below. Happy Holidays from the @SikoraLab! This year, @BordeauxFoSho...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...detection, treatment, and management. We are incredibly happy to hear that Gladys will be using plasmids in a ...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...

...blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs...capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya et al., 2016). In this case, location...location and sequence are of approximately equal importance in design – an optimized sequence will do ... using knockout or transcriptional modulation approaches. Avoiding off-target effects The off-target ...fully predict which sites will and will not show appreciable levels of modification. Whole-genome sequencing...

...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

... recent discussions of this apparently worsening crisis recently appeared in eLife: "A Fair Deal for PhD...news and updates. Scientists must do science to be happy. What do we have to change to ensure that all scientists..., there are some easy ways to get started. I am happy to work with any group of trainees interested in...

... is supposed to be impartial. The best science happens when scientists have 1) the courage to make mistakes...degree. It was a great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying...tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how to engage with friends...galilei-dialogues-concerning-two-new-sciences). This is the approach policy makers take in abusing climate science ...defund science and stay silent. I was overwhelmingly happy to see so many others come out who feel the same...

...through an electric field to deflect them into appropriate containers. The machine is useful for distinguishing...on in vitro studies where it was difficult to apprehend the key role of specialized cell types and organs...Good two-photon probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins...this requires other lasers than the standard Ti:Sapphire laser. It is also important to finely tune the...creation of mouse lines that can be used in fate mapping experiments. Immune response dynamics are much ...

...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept...

... a serious academic subject, it is now being appreciated on an aesthetic level as well. Beautiful microscope...can be used as a way to better understand and appreciate the organism, for both scientist and non-scientist...the difference in some areas of conservation.” Appreciating science as an art form taps into an emotional...matter is seen to have increased value and is appreciated by a wider audience for more than just its biological...

...for both transient and stable gene expression applications in plants. This smaller, easily customizable... susceptible to Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...only for plant engineering and biotechnology applications, but also for foundational studies of promoter...