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We narrowed to 535 results for: CAR

Showing: 261 - 280 of 535 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...genomic loci of interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector...sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...

  2. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ..., R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating that ...and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity...

  3. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...dendritic cells. These genetic tools were pivotal in carrying out my studies, and getting them from collaborators...has since studied and worked around the world, carrying out her undergraduate work at the University of...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    .... Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and...consisted of graduate students, post-grads, and early career scientists. Over the course of the class, the moderator...

  6. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... stone into the next professional stage of your career by connecting you with people of similar interests... research question that people (especially you!) care about. Have you critically evaluated your plan...

  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...wall synthesis Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal...optimal efficiency. Beware of satellite colonies! Carbenicillin is more stable than Ampicillin and can be used...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions using slightly different Cas... Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Jan;  ...

  9. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype ...

  10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ... new particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both ... possible, just to increase the viral vector’s carrying capacity. In order to allow the viral vectors ...

  11. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...pigments primarily found in plants in the order Caryophyllales, such as cacti and beets. Betanin belongs to... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy...

  12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...LTRs). After recombination, one resulting plasmid carries the transgene of interest (aka the viral genome...picking the smaller colonies which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed...

  13. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ... MBP tag can be removed using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag...for affinity purification (see plasmid map and cartoon below). Including biotin in your culture and inducing...

  15. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now... below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only the...

  16. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...institute where their typical workdays will be carried out until the end of the appointment. The visiting...don’t forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

  18. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...cell on their way to the nucleus where their gene cargoes can be expressed. The bottom line is that a lot...particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating your...

  20. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...larger. And since the library contains many plasmids, care must be taken to ensure that all of these plasmids...powerful research tool, but their proper use requires careful amplification and maintenance of good representation...
Showing: 261 - 280 of 535 results