We narrowed to 572 results for: CHI
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TypeBlog PostPublishedDec. 7, 2015, 6 p.m.... research, we hope we can help all researchers achieve happiness in their careers regardless of their ...by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find One? ...
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Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at...the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....complicated (and again, expensive) the different machines are, and how many regulations are in place to ... on the Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....resting state and its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor...inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....Alzhiemer’s disease (AD). Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....free interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific...Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to create gene-edited...claim has yet to be independently verified, the watching world erupted in controversy. Briefly, there are... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....you volunteer to do science outreach and teach children about science? Or perhaps you have presented in...specifically to assist graduate students in job searching. Start your career planning early, start now Don't... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m.... 3) produces bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results...however, you may need to run the gel for longer to achieve good separation of larger DNA fragments. I hope... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell.... 7th edition (2000). Meibom, Karin L., et al. "Chitin induces natural competence in Vibrio cholerae."... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m.... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....approaches. However, with each new technical achievement, the complexity of the device increases, as does...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...