Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 567 results for: CHI

Showing: 261 - 280 of 567 results

  1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to create gene-edited...claim has yet to be independently verified, the watching world erupted in controversy. Briefly, there are...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...complicated (and again, expensive) the different machines are, and how many regulations are in place to ... on the Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time...

  3. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can...

  4. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...free interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific...Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by...

  8. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...you volunteer to do science outreach and teach children about science? Or perhaps you have presented in...specifically to assist graduate students in job searching. Start your career planning early, start now Don't...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...

  14. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...

  15. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...approaches. However, with each new technical achievement, the complexity of the device increases, as does...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

  17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

  20. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...
Showing: 261 - 280 of 567 results