We narrowed to 578 results for: CHI

...119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5... AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...

...accompany him to YNP the following year. Geoff Schiebinger, another Quake Lab member joined for half of ...in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due to the 3,471 square...

...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...

...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research...

... research, we hope we can help all researchers achieve happiness in their careers regardless of their ...by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find One? ...

...things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at...the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent...

...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...

...complicated (and again, expensive) the different machines are, and how many regulations are in place to ... on the Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time...

...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can...

...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat...

...pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

...resting state and its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor...inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human...

...dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to create gene-edited...claim has yet to be independently verified, the watching world erupted in controversy. Briefly, there are...

...free interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific...Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by...

...was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects...

...you volunteer to do science outreach and teach children about science? Or perhaps you have presented in...specifically to assist graduate students in job searching. Start your career planning early, start now Don't...

... 3) produces bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results...however, you may need to run the gel for longer to achieve good separation of larger DNA fragments. I hope...

...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

...tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...

... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...