We narrowed to 514 results for: Cit

...Proteins can also be biotinylated by exploiting the specificity of the E.coli BPL, BirA. If you recall, BirA ...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b...

... essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to ...powerful boost to genome editing experiments. We’re excited to see how you continue to use and develop AAVs...

...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or break your...

...described in 2013 (Cong et al, Mai et al) we were very excited to set up a CRISPR-based editing platform in our...study what’s wrong with the cells. However, our excitement turned into frustration when we sequenced our...

...hypothesized that genes that had evolved specifically for toxicity would allow stronger counter-selection than one...an efficient allelic exchange system, he is so excited to use it to explore how hospital-associated Gram-negative...

... Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins...the reaction, or have difficulties with kinase specificity in vitro. The establishment of a system in which...

...skipping approach in mice, DMD researchers are very excited. This approach may also be applicable to a number...the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized...

...type depends largely on the viral capsid, but specificity doesn’t end there: expression from rAAVs also...Transduction but Differ in Sialic Acid Linkage Specificity. Journal of Virology, 75(15), 6884–6893. https...

... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...

...treatments for external companies. It was really exciting to work in a larger commercial company; the pay...opportunity to work in a genetics lab. As a UK citizen, I was entitled to a Work-Travel VISA, which means...

...of knowledge, experience, and new professional capacity generated during this process result directly ...mentors and mentees). This is sure to be another exciting year for IMP! Many thanks to our guest bloggers...

...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...shown to offer high activity and unparalleled specificity in genomic targeting design flexibility. The ...cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently ...

...detect specific nucleotide sequences with high specificity and quantitative accuracy and even include profiling...PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of the nick-closing activity of bacteriophage...

...change. This method has high sensitivity but low specificity (Degeling, 2012). Bioluminescence assay kits ...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

...original enzyme, SNAP-tag and CLIP-tag show good specificity for their own ligands with minimal cross-reactivity...Protein-based tags typically provide better labeling specificity than short peptide tags like tetracysteine (Jing...

...parallel so you have a good measure of ICC assay specificity. We generally include Sox2-V5 tagging as a positive...activity of CRISPR reagents and ICC antibody specificity. Derive clonal lines by single-cell deposition...

...final plasmid pages and scientists will be asked to cite this article when using your plasmids. You can submit...Deposit Spreadsheet More Data for You: Find Articles Citing Addgene Plasmids Resources on the Addgene Website...

...pattern of labeling, this is supportive of their specificity for the protein. A challenge to this approach...target with very low expression, due to induced toxicity caused by the protein of interest. HLDA workshop...

... Their trust in the quality of the content is implicitly defined by their trust in the piñata builders...24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology...

...other techniques all aim to improve targeting specificity or reduce the duration of Cas9 activity.  The...recognize the same PAM and had similar targeting specificity as full-length Cas9 (flCas9). When triggered ...