We narrowed to 379 results for: ENA;

1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether...

2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic...

3. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome...

4. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   Published

   July 14, 2021, 1:15 p.m.

   ...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...

5. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

6. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...antigen tend to work very well for applications that denature a protein to its primary (linear) structure using...

7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further ...

8. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian ...

9. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ... of the biomedical research sector, and what a tenable workforce structure may look like. The panel discussion...

10. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...multiple internal restriction sites that may not be amenable to "domestication", you might want to consider...

11. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...where I dump all of my papers and they all have filenames (year first author name). It’s rudimentary, but...

12. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...of all organisms depends on efficient energy maintenance - through acquisition, storage, and utilization...

13. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...deletion). Find the Plasmids Here Plasmid features enable multiple student experiments We used TOPO cloning...

14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...Natural History Museum has downloadable resources to enable you to set up your own citizen science project....

15. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ... of a protein in a synapse. What kinds of tools enable us to measure these properties, and what fascinating...

16. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ... Doench, J. G. (2024). Modular vector assembly enables rapid assessment of emerging CRISPR technologies...

17. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ... stripping solution uses heat and detergent to denature and remove antibodies but is also likely to remove...

18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant...

19. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ... "The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans...

20. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...you can start by asking ‘What is the biological phenomena I want to observe?’ This information, along with...