...every amino acid I’d like to encode,” but, as described above, not every region of a protein should necessarily...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

...genome editing with CRISPR/Cas9 in human cells was described in 2013 (Cong et al, Mai et al) we were very excited...thinking further about the distance relationship described above, we realized that, in addition to increasing... and neurovascular diseases. He worked on the described project while he was a Postdoc in Marc Tessier-Lavigne...

... through Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits..., Cao Q, Zhang Z, Ding Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus as versatile expression....2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation. Nature 532:293–293 . https://doi.org...

...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by ...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3... optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of ...

...promoters can be used in the repressible manner described above, as well as the inducible manner detailed...30 years since the original Tet-Off system was described by Gossen and Bujard, both the promoter and transactivator...cells, and can be used in the repressible manner described above, as well as the inducible manner detailed...

...for and how can you do it well? Below, I briefly describe some tips for a successful postdoc fellowship.... there a tractable question or are you simply describing a phenomenon? Exploration can be important, but...in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell imaging approaches. ...

... been described in this blog. If your data does not meet the assumptions for the tests described above...error rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...

... community is generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed...videos and text on the website for more detailed descriptions of how to use the software. Here is a brief ...sequences, and more. Output_Compact: A text file that describes which genes were included, and how many gRNAs ...

...like AAV. In addition to the mammalian option described below, plasmids for making polycistronic gRNAs...oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does not contain Cas9, so you... are similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease...

...AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com/pYBL3aLK4f...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape...

...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...

...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...phenomena and about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments...

...researchers at Japan’s Hiroshima University who described their new tool in Scientific Reports in June. ... gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction system...

...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started!  I learned...

...activated and can cleave fluorescent probes of dsDNA and RNA in trans. Fluorescent signal indicates the presence...

...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique to screen for...

...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,...

... Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids...