Skip to main content
Addgene
Showing: 261 - 280 of 377 results

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA...

  6. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...molecules by conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867...

  8. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

  10. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

  12. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...strand synthesis. In contrast, a scAAV vector with half the size of the ssAAV genome has a mutation in the...

  13. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...Technical difficulties turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’re more ...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...cavity of Cas12f. The CasMINI system is less than half the size of the Cas9 or Cas12a based systems, making...

  18. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (2022) A guide to preprinting for...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...
Showing: 261 - 280 of 377 results