We narrowed to 402 results for: LARGE;

1. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...effectively a million dollar sales pitch. Given this large sum, one might expect that applicants would work...

2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...about the advantages of pooled CRISPR libraries for large-scale functional screens Read this blog post about...

3. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ... purification procedures. Even if you produce a large amount of protein in your expression host of choice...

4. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...

5. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes...

6. How to Write a Scientific Review Article

   Type

   Blog Post

   Published

   Feb. 16, 2017, 3:30 p.m.

   ..., to search the literature, and to synthesize a large volume of information: basically, a scientist’s ...

7. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction enzymes needed Not...

8. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...

9. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ... vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out...

10. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...from purified recombinant proteins at small and large scale.  This new technique involves selection in...

11. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral vectors, they...

12. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...software was originally designed to help us work in large formats, such as 96-well microplates, and thanks...realized the need to modernize our approach to handle larger volumes of data. In this two-part blog series, ...

13. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ... and structure. Their porous surface provides a large area for binding, but antibodies conjugated within...

14. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...

15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ...

16. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ... did, and state that it would help you manage a large number of files as part of the job. Structure of...

17. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...variants use different RT enzymes and performed large edits with similar or better efficiency than PEmax...

18. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized...

19. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...outlined below, allow for researchers to select large sets (with overhangs that violate some of the traditional...

20. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...