We narrowed to 321 results for: Lor
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TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....more than one right answer, so don’t be afraid to explore multiple approaches to find out which one works...
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Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....cloacae, a bacterium that’s part of normal human gut flora. Guides targeted essential and non-essential genes... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....cassette in one single viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas delivery... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....articles have been filled with impressive, full-color images of proteins. But they are still flat. To ... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....manager to gain experience in the area I want to explore while also ensuring that my work gets completed... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m.... JFs can cross the blood brain barrier and many colors of JFs are available to swap into the system. Single... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m.... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use of MMEJ represents... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....State of Science could be a jumping off point to explore future rabbit holes. For the non-scientists in ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....Cancer Institute, and Koch Institute, where an exploration into the effects of ancestry and germline sequence... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m..... PubMed Central PMCID: PMC355630 Faras, A. J., Taylor, J. M., Levinson, W. E., Goodman, H. M., & Bishop... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....have even considered. These questions should be tailored to the group and format but should almost universally... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....better together.I realize I may have painted a rose-colored-glasses-esque picture of how everyone is kind and... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....effects of guide length on off-target cuts was explored using full-length (19-20 bp) or short (17-18 bp... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m.... fresh set of erasable markers. You’ll have the colors you want and you know they will work. Stabilize... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA (2019) CasX...