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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 662 results for: MAL

Showing: 261 - 280 of 662 results

  1. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...stable gene expression applications in plants. This smaller, easily customizable vector functions in multiple...plant scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity would...infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially available pCAMBIA and pKYLX vectors...

  2. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...club of people who have been in space is pretty small). Therefore, I was delighted to be invited to the...important part of their work? First, the discovery of a small, programmable DNA cutting enzyme. Second, since ...discussed how J&J is already modifying cell and animal genomes to test hypotheses of disease. They are...

  3. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...in a genetically encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein...Schwarz-Linek at St. Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking... types, like stem cells or cells infected with malaria or other parasites. Addgene: Do you have any particular...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...create SpCas9 base editors with editing windows as small as 1-2 nucleotides. To reduce off-target effects...Another way to improve base editing efficiency for mammalian edits is to ensure the editors make it into the... CBEs (called Td-CBEs, CBE-Ts, or TadCBEs) are smaller and less likely to cause off-target mutations than...2023). Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target...

  5. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...efficiency for point mutations and 66% efficiency for small insertions. Multiplexing of two targets simultaneously.... coli; while the Marraffini lab used a native minimal CRISPR array (Bikard et al., 2013), the Qi lab ... plasmids Stanley Qi’s lab demonstrated that a minimal, regulatable CRISPR system effectively silenced...transcription of one or more genes in E. coli. and in a mammalian cell line (Qi et al., 2013). Two important features...

  6. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...method is that it can be performed in a live awake animal by surgical implantation of the probe into a specific...powerful and offers immediate measurements in a live animal that can be correlated to behavior, the technique... molecular tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and...

  7. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...specialized Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste...editing was initially described by Cong et al. and Mali et al. in January 2013 (7, 8). Nine months later...Zhang F. Science. 2013 Jan; 339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE...

  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ... Step Further - Big Company v. Small Company Big (e.g. Pharma) Small (e.g. Biotech) Develop expertise...ride Complicated matrix structures are common Small integrated teams, little hierarchy Real diversity...

  10. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ... experiments. Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from... A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly used... MIT) shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments...

  11. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...a DNA sample into many small components. Without proper processing, these small DNA strands can form concatemers... produced high yields of quality libraries from small amounts of DNA i.e 100 pg. At the start of the workshop...

  12. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...portion of the complex and is derived from the malachite green (MG) dye, but has been modified with the...et al also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed to YFP ...Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org ...

  13. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ... opportunity to advance, and how many jobs are normally available (as sectors such as forensics currently...the relevant skills and experience.You might not normally think about it, but hobbies and volunteer experiences...are willing to exercise these skills outside of a formal work environment also shows that you are passionate...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...based multicolor labeling system to visualize chromosomal loci in human cells (Ma et al., 2015). To do ...aptamers are nucleic acid sequences that bind to the small molecule, DFHBI-1T to induce its fluorescence. They...Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proceedings of the National...

  16. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...MBE) and multiplex prime editing (MPE) with only minimal payloads. DAP array  Although CRISPR-Cas12a, with...advantages in gene editing, while keeping the system to minimal size. To do so, they harnessed the tRNA processing...any cell type or organisms of interest with only minimal genetic payloads. Different from existing multiplex...

  17. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... biological processes. Chemical effectors are suboptimal for a number of reasons, including their potential...number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter ...transcription factors, for which low expression levels are optimal. These tools are compatible with many strains of...

  18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved folding...however, that there is no single fluorescent protein optimal for all organisms and that if you want the brightest...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL... Hudry et al, 2023; Hinderer et al, 2018). The optimal dose for each particular experiment is determined...validate the capsid for your specific application and animal model.  Other model organisms Tropism and efficacy...transduces BALB/cJ, CBA/J, and FVB/NJ mice, with minimal liver transduction observed in both BALB/cJ and...and AAV-PHP.S, with a bias toward segments of the small intestine (Chen et al., 2022). Systemic capsid for...

  20. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... BioBuilder experience With its intentionally malleable curriculum, BioBuilder takes cutting-edge research...runoff from winter icing.BioBuilderClub is the only informal synthetic biology club of its kind, running from...who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory kit...
Showing: 261 - 280 of 662 results