We narrowed to 469 results for: NOL
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TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....pluripotent stem cells from human keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed PMID: 18931654... cells from human pluripotent stem cells. Nat Biotechnol, 2014. 32(1): p. 84-91. PubMed PMID: 24291815...
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CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red and Cas9, which...for highly specific genome editing.” Nature Biotechnology 32(6) (2014): 569-576. PubMed PMID: 24770325... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....have been making major improvements to CRISPR technology since its initial applications for genome engineering...CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol 33:755–760 . https://doi.org/10.1038/nbt.3245... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m....blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A ...editing, regulating and targeting genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m.... PubMed PMID: 28585549 Kim Y, et al. Nature Biotechnology. 2017. PubMed PMID: 28191901 Listen to Our ...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....membrane, you can dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins...the bubbles in the membrane sandwich Ensure methanol concentration in transfer buffer is < 20% ... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....working in fly research, I hope you find this new technology as helpful as we have! References and Resources... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m.... a laughter program significantly decreased immunological markers of stress in adolescents. It also put... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog PostPublishedSept. 5, 2018, 12:01 p.m.... students to this amazing genome enegineering technology Resources at Addgene.org Find basic molecular... -
The Codon Guide to Synthetic Biology
TypeBlog PostPublishedSept. 14, 2023, 1:15 p.m.... at MIT. He also writes Codon, a blog about biotechnology. ... -
Introducing Addgene's Microbiology Pages
TypeBlog PostPublishedJune 13, 2016, 7:08 p.m....microorganism, microbial technique, or microbial technology, sign up to write about it on the Addgene blog... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m....Sciences at Missouri University of Science and Technology, has been teaching the microbiology lab every... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m....Scientific Sharing: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data Freedom: ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog PostPublishedNov. 29, 2017, 2 p.m....investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising... -
MassBioEd Career Exploration Day at Addgene
TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....presentation on the emerging CRISPR genome editing technology was a rousing success! Many of the students were... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m....that have completely student/postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog PostPublishedJune 26, 2014, 2:23 p.m....purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica spin column... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m.... understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious pace. ... -
Microbes: Look, Listen, and Tell
TypeBlog PostPublishedAug. 28, 2018, 12:04 p.m....vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....variety of pooled libraries. Not all of these technologies were without controversy, and it remains to ...