Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 729 results for: NSI;

Showing: 261 - 280 of 729 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...have, and we developed a great plan to start consistently knocking out high-quality videos for you all...two options: Film outside at a distance, or film inside with one person in a room. I wouldn’t be in there...live. After doing a bit of looking, I found an inexpensive HDMI transmitter that could transmit a signal... stand and mounted a monitor to it. It had an extension cord attached, with the option to mount a battery...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...called a katana. Opto-katanin consists of two separate blue light-sensitive systems, VVDfast to control ...switch. You may be familiar with popular light-sensitive ion channels used to control activities like neuronal...in other contexts are sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins...

  3. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... clients focus on their experimental needs and consider a budget as a resource checklist. A budget is ...centrifuges, sequencers and analyzers, and any ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes...awry when several factors aren’t taken into consideration. 1. Always focus on the science When negotiating...However extreme a solution may seem, you should consider it if it will help you achieve your research goals...

  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...first.  AAV-mediated gene expression can differ extensively depending on the experimental system used, so...Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could help, or harm, your experimental...occur (Fig. 3) - for example, some neurons can transiently express TH - so you’ll need to check for this...injection volume.  There are a few factors to consider. First, the titer required is inversely related...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... have engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1 promoter...systems. Binary systems are genetic tools that consist of two parts, both of which must be present for...results in chimeric GAL80-repressible and GAL80-insensitive activators of lexAop. This system is commonly...expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun...

  6. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...the results across runs. If the results vary considerably, then the high degree of variability in the ...assays have their own unique controls that must be considered (Burry 2011). Perhaps the most critical are those...materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit less ideal...signal remaining is autofluorescence and should be considered in the interpretation of results. Include the...

  7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ... sequencing would be too expensive, then the qPCR method should be considered as an alternative. The qPCR...three-input GRSMs, we recommend using overlap extension PCR and Gibson assembly to piece together the ...circuits in general would benefit from a more comprehensive characterization of the recombinases at our ...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...encode a transposase enzyme.  Class I TEs are considered replicative since every time they jump they make...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has ...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Tol2 is only active in vertebrates ...site preference and host range are important to consider when selecting a transposon for a mutagenesis ...

  9. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...Application Recombination Frequency Special Considerations dsDNA Insertion or deletion of >20 nucleotides...CI. For recombineering purposes, a temperature sensitive version of the repressor gene, CI857, is used....express lambda red genes from a plasmid with a heat sensitive origin of replication.  Once the recombineering...expression of the int and xis genes which are responsible for excision of mini-λ from the host’s chromosome...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...wherein multi-part assembly can occur. It is quite extensive with a supplemental add-on kit and is compatible...William M., et al. “A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae....pone.0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library for Advanced...2024.02.16.580396. Martella, Andrea, et al. “EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid...

  11. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...life-saving research are being proposed.  It's our responsibility to join the public conversation and to tell...scientists – including global warming skeptics – insisted that more data and research funding are critical...towards starting these conversations; it is our responsibility to continue the dialogue. Erin Golden is currently...continue to worry) that most who participated will consider that marching in and of itself will be enough....

  12. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...and understanding of CRISPR, and perhaps provide insights and ideas for your own research! Classification...with BioRender.com.   Type III Type III is considered the most complex of the CRISPR types and is thought...et al., 2021). IV-A and IV-B lack what you may consider to be an integral component of CRISPR — a nuclease...2021). Evolution of Type IV CRISPR-Cas Systems: Insights from CRISPR Loci in Integrative Conjugative Elements...

  13. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ... with “A Better Way to Share Science” for: The inside scoop from scientists who have deposited plasmids...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.  ...

  14. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...Listen to this episode for insights into the process of getting an MD PhD, the inside scoop on developing new... MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently became a member of ...

  15. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...and on-targeting likely more efficient.  These insights are hard to appreciate without computational support...tools The goal of this post is to provide some insight into available CRISPR software tools, what problems... software tools that apply some approximate or sensible rules to determine the value of one sgRNA design...functions are not entirely necessary but should be considered extremely useful, depending on design goals. ...

  16. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...transformation into Sample 6 and Dr. Koeris' own transition from graduate student to entrepreneur, listen...founding a startup company? 32:20 - 34:37 : Whose responsibility is it to take on the risk of developing products...37-36:53 : Was It difficult for Dr. Koeris to transition from developing therapeutics to developing a ...

  17. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been... critical for viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows...

  18. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...quoted as saying when the news first broke. "Considering that many human diseases are caused by genetic...presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography, an advance they... and mainstream the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes...

  19. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...compiled our top career blog posts into one comprehensive, downloadable guide: the Addgene Science Career...you’d like to see discussed on the Addgene blog, consider writing a guest post. Additional resources on... Find job listings at Addgene Get more career insight from Addgenies ...

  20. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported its...hypothesized that GFP-like proteins could be responsible and began studying these marine organisms to ... fell into place, as they, taken alongside the insights provided by the studies in Tsien lab, covered ...
Showing: 261 - 280 of 729 results