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We narrowed to 789 results for: OMP;

Showing: 261 - 280 of 789 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as pichia). What...general research community. Phillips Petroleum Company first commercially developed K. phaffii in the ...Since then, multiple biotech and pharmaceutical companies (along with some research laboratories) have picked...of the uses outlined below. Biology They say comparison is the thief of joy, but I think it’s acceptable...10.3389/fmicb.2020.607028  Riley, R., et al., Comparative genomics of biotechnologically important yeasts...

  2. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...equivalent of crossing off days on the calendar after completing your writing goal, which can serve as positive...graph of which days of the week you most often completed your habit is useful for figuring out when to ...makes suggestions on how to make your writing less complex. While I don’t think you should accept all of the...jargon in your writing. While it’s not possible to completely avoid jargon, this tool can help you avoid overusing...collaborators. Zotero is a desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...Most of our equipment has been donated by local companies and BosLab has grown into an organization consisting... Timothy Stiles joined BosLab last year with a computer science degree and had zero pipetting experience...yeast to glow, produce synthetic scents, detect compounds in the environment, and openly publish protocols...the local community (including local garden and compost clubs) to participate in this study. How to get...

  4. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ... other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm...SYBRGreen, are specific for double strand DNA (as compared to spectrophotometric methods that measure all... corresponding fluorescence. That way, you can compare the fluorescence of your sample against this curve...relative to the intensity in the ladder. It’s best to compare the band intensity of the fragment in the ladder...sugars under acidic conditions and forms a blue complex that can be quantified at 595 nm. This method is...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... precision gene editing. CRISPR-Cas9, the key component to enable efficient base editing and prime editing...unable to process its own array but is the key component to enable efficient base editing and prime editing... or any other kinds of CRISPR tools, without compromising their strategic advantages in gene editing, ...higher Cas9-independent off-target editing in comparison with single-site editing. Encouraged by the results...#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

  6. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...need to be decided. Figure out what you need to accomplish to get to the next stage, and then adjourn. ... if any additional resources are needed. All-Company Meetings: As your organization grows, so will the...to address one point aloud. This keeps our all-company meetings short, informative, and pleasant. Email... positive peer pressure to make sure everyone completes their assigned items to keep the project moving...timely feedback on each other’s projects. For all-company meetings, monthly still works best. Figure out ...

  7. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... You can do more than just ‘sense’ biological compounds and reactions; you can quantify them with biosensors... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in... that you are interested in. The most obvious component of a biosensor is the thing that you care about...or an electrolyte such as calcium. This varied component, which we will refer to as the analyte, needs ... fluorescence, pH change, heat, etc. The last component of the biosensor is the signal transducer which...

  8. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...researchers new to GCE aren’t able to successfully complete their experiments and many others are dubious ...’t give it a try. Optimization Why is GCE so complicated? Well, while the techniques are straightforward...tools are not very robust. When using GCE, each component of the experiment system (including media, cell...optimization parameters the same for all three components, meaning that the final protocol may involve ...has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan Mehl would know. He’s the director...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cas9.  For a brief comparison of Cpf1 vs. Cas9, see the table below. Table 1. Comparing the Cas9 and Cpf1...simplified system for editing multiple targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing...Cpf1 CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size ...expression vector.  See the graph in figure 2 for a comparison of editing frequency resulting from transfection...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...Anti-FLAG frankenbody (FB) construction from the complementarity determining region of an anti-FLAG scFv and... variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes when... of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple...CRISPR-Cas system for editing and gene activation, is composed of only 529 amino acids, less than half the size...or Cas12a. Despite its small size, CasMINI is comparable in efficacy to dCas12a systems in both gene activation...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...another way to activate gene expression, and two-component systems used in synthetic biology use light to... Addgene also has popular light-regulated two component systems designed by the Tabor lab. Which inducible...crossover. The induction mechanism must also be compatible with your experimental system. Leakiness If you...Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other blog posts from...

  12. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Cas enzymes and variants available. Without a comprehensive resource, it can be overwhelming to choose a...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...how can I multiplex this enzyme? What other RNA components are required? CasPEDIA provides information on...gRNA design, etc.). With this feature, you can compare different Cas enzymes to make sure you are getting...isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...be incorporated into these constructs without compromising cleavage efficiency. In addition, tRNAs contain...generation for two PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs...examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs. In PTG ...frequency at both targets (35% and 76% biallelic), compared with only 44% and 66% indel frequency with the...in the graphic below - including some simple comparisons. The easy multiplexing afforded by PTGs will...

  14. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity Empty rAAV has a cargo...conventional single-stranded AAV (ssAAV) and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector...2.5Kb) and you need expression in a hurry, self-complementary AAV (scAAV) may be an option (McCarty et al....take the next step – selecting your AAV and AAV components (serotype and promoter) for use in your experiment... E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... changes when it goes from a more neutral pH compartment, ­­like the cytosol, to the higher pH of the ... to other pathways, it is standard practice to compare degradation rates between samples treated with ...the brightly-coloured Australian parrot. It’s comprised of two tandem fluorescent proteins: a relatively...Rosella’s excitation and emission spectra. Component Excitation Emission pH Range DsRed.T3 488, ...autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy, the targeted autophagy...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...CasMINI plasmids Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs Yong-Sam Kim...result is a highly efficient Cas system with very compact genome editors and a gRNA down-sized by almost ...activity of Cas12f1 with these modified gRNAs is comparable to that of SpCas9. The group also confirmed that...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions...enzyme with a fully active Cas9 enzyme. PE-Cas9 comprises an active Cas9 fused to the reverse transcriptase...

  17. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...multi-protein complex, stabilized promoters can eliminate variability between the different components. When ... origin of replication will be overrepresented compared to those further away. As a result, variable gene...located near the origin will be over-represented compared to those further away. While constitutive promoters...gene expression during shifts in growth rate as compared to a constitutive promoter. To simulate the increase...

  18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ...SpCas9-HF1 generated fewer off-target cuts when compared to WT SpCas9 across a variety of genomic sites...M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased off-target...off-target activity across the sites tested when compared to eSpCas9(1.1) and SpCas9-HF1. The HypaCas9 plasmids... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...

  19. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ... The destination vector contains sites with complementary overhangs that direct assembly of the final ...recognition site and permits DNA fragments with compatible overhangs to be efficiently assembled. Scientists...inverse orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled... reaction. For detailed Golden Gate protocols, complete with helpful tips and tricks, see The Sainsbury...system is an incredibly powerful tool for cloning complicated constructs in a single, high-efficiency step....

  20. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...logical progression of your work, and you can accomplish this by employing a neat and organized format...length, and will be more difficult to read. The Thompson Rivers University Library provides useful tips...want to make sure that the color of your font is compatible. Use high-contrast between these colors so that...
Showing: 261 - 280 of 789 results