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We narrowed to 847 results for: PLE

Showing: 261 - 280 of 847 results

  1. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...research project can take several years to complete and has multiple benchmarks. Think about what your personal...professional stage of your career by connecting you with people of similar interests and goals. The following questions...field? You want to choose a research question that people (especially you!) care about. Have you critically...reach these milestones? Can you get assistance from people who have the required expertise to help move your... of keeping up-to-date with the literature of a couple of well-known journals that discuss general science...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

  2. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...not powerful enough for large brain samples. For large brain samples, we recommend pretreatment with ScaleCUBIC1...fluorescent protein (XFP) genes in a transgene. When multiple copies of the transgene cassette are introduced...resulting in low fluorescent protein level. The principle of Tetbow In modern neuroscience, plasmid or viral...time points, sacrifice the animals and fix the samples. When you use AAVs, it typically takes a few weeks...excessive amount of XFPs expressed. Then, clear the samples and visualize. For more details on this procedure..."Optical clearing and index matching of tissue samples for High-resolution fluorescence imaging using ...

  3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...transparently reveal them upon completing an experiment. There’s not necessarily in-principle acceptance, but readers...simply doing an experiment wrong. However, even simple procedures like pouring a plate or running a gel...sifting through paper lab notebooks). Once you’ve completed your experiment, you’ll need to analyze your results...individuals and by organizations like Addgene (a good example is our series of blog posts on the defunct genome...promote reproducible publishing practices. One example is the publication of peer reviews so we can see...and, if approved, the researcher would get in-principle acceptance of the final manuscript regardless ...interlab peer review of experimental design. These are simple steps but could go a long way to encouraging researchers...

  4. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...while working remotely. Social distancing? Many people are now calling this “physical distancing” since... important to stay virtually connected with the people in your life. At Addgene, we’ve started virtual...SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing. Social...take-out) or volunteering to foster a pet, for example. If you are looking for some resources specific...activities that kids can do more independently. For example, they could do something like puzzling or crafts... time.  Find learning activities for kids. For example, Skype a Scientist is now open to families at home... future experiments and other researchers by implementing reproducibility best practices in your research...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR... bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR technology becomes increasingly...using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting this post...look at CRISPR’s potential for use in treating a complex genetic diseases, Alzhiemer’s disease (AD).  Troy...diseases with real phenotypic effect.” “It’s a very simple technology,” Doudna further explained. “That’s ...  Multiple Authors...

  6. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...the requesters what they needed. In the example above, for example, we fostered a formal scientific collaboration...uploaded to the preprint repository BioRxiv. This example clearly illustrates the power of collaboration,...choice but to quarantine in our houses, feeling helpless about the situation. But a few days later we had...certain projects. The idea behind the platform was simple: any person working on COVID-19 could file a request...groups. In other cases, the collaborations were simpler and the volunteers helped with things like translations...need help with your scientific research. It’s completely free, of course. You can find more information...

  7. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...where we aim to do large scale DNA preps with multiple samples without cross contamination. We currently ... in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes...futuristic with block chairs in the hallway for people to take a break and relax. Companies at LabCentral...create a DNA library, one must first fragment a DNA sample into many small components. Without proper processing...process with, we got great amplification of our sample libraries. NEB also gave us a few goodies which... I am bombarded with a lot of information. For example, before going to my next workshop, I’ll ask myself...

  8. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...realized that it was easy to find people who were interested. For example, our journal club discussion on... CRISPR/Cas9 gene drive paper was very popular. People kept asking if we were teaching classes at the ... safety training prior to getting started. For example, Timothy Stiles joined BosLab last year with a ... space to perform early-stage experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab... getting the community to collect various soil samples, extract DNA, sequence it, and analyze the data... characterize the microbiomes of the different samples. The goal is to not only learn something about ...

  9. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes ...the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically study gene ...downstream of a tissue-specific promoter sounds simpler, this modular system saves you from having to create... making a fly line in the next blog post). For example, the hedgehog gene can be overexpressed in several...under the control of UAS enhancer sequences. For example, if you discovered five new genes and wanted to...control purposes that there are dosage effects. For example, overexpression of two genes by one Gal4 driver...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...understanding these complex processes can’t be overstated, we can also learn a lot from Nature’s simpler solutions...engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly ...These questions can be addressed using R bodies: simple, force-generating protein machines that can extend...could be used in a variety of applications. For example, you could use them to lyse cells (and surrounding...

  11. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...time for that and you won’t be as productive. For example, the many resources available on the Addgene website...less dependent on the manager to succeed. More people in leadership roles leads to more ideas, initiative...enough to do…you are not delegating enough. For example, if we need to develop a new plasmid collection...duplicated. With each component of the project completed by an individual who is a local expert in that...result is high quality and the entire process is completed more efficiently. Listen to our podcast interview... Don’t give out tasks – where possible, include people in deciding what is to be delegated to them. Not...your time and skills. It also ensures that other people in the team grow and develop to reach their full...

  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...being one of the five senses? GPCRs or G-protein-coupled receptors (GPCRs)! Aside from these three senses...cascades.  So let’s break down the name "G-protein coupled receptor" into two parts: the receptor itself and...Takeda et al., 2002). G proteins: The receptor is coupled with heterotrimeric G proteins on the intracellular...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...finally, to a response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the human genome sequence. ...

  13. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ... to determine precisely how the methods were implemented. We run into this problem frequently over the...not robust. Sampling noise There is, of course, simple noise in the process of sampling and inference-...problems above: unclear methods, QRPs, and small samples, then when replications do not come out the same...statistical tools that generate a p-value.  As an example, if you hypothesize that growth rates will be higher...answer those questions, the article is given “in-principle acceptance” to publish the final results regardless... special emphasis on positive controls and good sample sizes, concerns that are too easy to overlook when... worked with citizen scientists to design and implement authentic scientific research and as the Director...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Chemical agents, temperature, and light are all examples of factors that can lead to the induction of a... these three types of inducible promoters, and examples of each type. Many of these promoter systems are...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP ...represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters include positive...in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated...temperatures but can be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90...

  15. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... we've heard over and over from our users: "Please, please improve your plasmid maps!" After thoughtful...just how much things have improved, check out the example below. Our old map is on the left while the SnapGene...on to learn more about the improvements we’ve implemented and how they’ll make it easier for you to find... next cloning experiment early on. Beyond these simple but powerful display improvements, when you click...or ORF to learn more information about it. For example, if you hover over the ampicillin resistance gene...software development teams for working tirelessly to implement these improvements. Let us know what you think...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter... data gathering, regulatory efforts, policy implementation, program management, communications, diplomacy...setting: drafting legislation, making rules, and implementing the policy within the jurisdiction of the governing...working in science policy doesn’t have to be a complete career pivot. There are many ways to pursue your...your interests in policy or advocacy as a supplement to your career through engagement with your local...proposals. Check out the Day One Project for some example proposals and consider applying for their accelerator...

  17. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ...techniques. These include reusing pipettes for multiple cell lines rather than using disposable ones or... of powdered media. Filtration does not ensure complete sterilization as mycoplasma can even escape 0.2... growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture, it...by providing proper training and guidance to the people working in the lab facility. This can be done by...mycoplasma infection from one cell line to another. Multiple cell lines should not be stored in a single incubator...cultures. The treatment can take a few weeks to a couple of months depending on the severity of the infection...

  18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...disease estimated to affect 0.27 to 4.6 per 10,000 people globally. Pustules form from the uncontrolled activation...where one of the X chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic... mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the...translate those signals into cellular responses. For example, GPCRs and their downstream G protein partners ...with an emphasis on oligomerization, signalling complex assembly, and localization to the nucleus,” says...at McGill University.  These protein-receptor complexes consist of many parts that work together on the...gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental delay, and...

  19. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...’s scientific seminars a couple days a week. And there there’s always plenty of writing: plain-language...social behaviour in social insects, obviously as all people are,” says Susan. Her passion for insects lead ...show. After graduating with a master’s, Susan completed a science writing internship with the National...planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications and ...the most satisfying parts of the job is having completed one of these difficult pieces: “It’s probably ...starting a blog, but with one caveat: “I hate telling people to work for free because you shouldn’t work for...

  20. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...recent advances have allowed scientists to track multiple genomic loci over time using all the colors of...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building on the work of...al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful ... Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However, they ...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...
Showing: 261 - 280 of 847 results