We narrowed to 552 results for: PPL;
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TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....of xanthine into guanine, and without xanthine supplied in the media, these bacteria are unable to divide...was able to grow this strain in minimal media supplemented with various caffeinated beverages, but not ...
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Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....it comes to targeting precision and practical application. If the CRISPR/Cas9 system is not effective for...lncRNAs with high specificity, particularly in applications requiring smaller delivery systems (Zibitt et... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m.... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....the co-op application process, my advisor encouraged me to keep my values in mind as I applied. Thinking... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...and who will be affected by the knowledge and applications generated from this research. At both a practical... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....focused on developing cell-free synthetic biology applications for human health and the environment. Alexander...nanomaterials science, and self-assembly for applications in health, energy, and sustainability. References... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m....eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....the initial laser output. The same considerations apply to the polishing and preparation of the patch cable...Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science. 2013;340... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m.... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....and respond to novel substances. We both had an applied science bent, so the engineering aspect of this...about continuing with the business. We ended up applying to a business accelerator freely stating that ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....machine learning (PRIDICT, PRIDICT2.0). How to apply PRIDICT in prime editing experiments Prediction ... computational biology. His expertise includes applying machine learning techniques to make sense of biology... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment! ...Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https:/... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since...Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer therapy. Elsevier, pp ... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...