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We narrowed to 781 results for: Por

Showing: 261 - 280 of 781 results

  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... This is important to ensure that you’re looking at institutes that have the means to support your work...screening out irrelevant candidates, and, more importantly, it’ll help you define salary costs. 4. Ascribe...make these decisions in the following order of importance: 1. First make decisions based on experimental...and availability of the equipment is of utmost importance. Conducting a successful lab start up negotiation...

  2. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes... mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes...Allows uptake of large plasmids hee   High electroporation efficiency supE44 (glnV44)   Suppression ...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F-mcrA Δ(mrr-hsdRMS-mcrBC...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et al., 2014), and postsynaptic ... system, remember that some serotypes can be transported retrogradely and/or anterogradely along the axon...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse...Favre, D., Blouin, V., Provost, N., Spisek, R., Porrot, F., Bohl, D., Marmé, F., Chérel, Y., Salvetti,...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... homology arms and a selection marker, then electroporate it into mouse embryonic stem cells. After selecting...compatible with standard microinjection and electroporation methods using in mouse transgenics. Designing...between the ampulla and infundibulum. In vivo electroporation is performed immediately after injection. Image...tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...

  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...engineer cellular processes that are influenced by temporally ordered biochemical events. How do RSMs work?...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...

  6. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...editing technology led to the development of many important medical and agricultural products. But it also...Ridley Scott wonder what would happen if these corporations would extrapolate genetic engineering to exploit...which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases In 1989 the...SciFi is one of the most imaginative and most important genres of literary fiction. It has always served...health sciences, energy, and agriculture, its portrayal in popular fiction has been largely negative. ...

  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ... drove work in countless other systems, whereas import of CRISPR technology into C. elegans has been a...

  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...looking through a paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash...itself when looking down a paper thin and very portable microscope. Less fluorescence admittedly, but ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...

  9. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...provided by the studies in Tsien lab, covered a portion of the color spectrum that GFP and its derivatives...reach. Nobel Prize for fluorescent proteins The importance of fluorescent proteins was recognized in 2008...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...number of cells needed is lower, and perhaps most important, targeting all genes doesn’t necessarily add value...cytokine known to cause the up-regulation of an important surface protein (e.g. PD-L1 on a cancer cell)....are migratory, and their exact localization is important for their function. Their migration is induced... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

  11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...impossible to determine specific protein turnover or temporal expression using standard FP-chimeric proteins...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...localization, or leading to protein aggregation. Another important parameter to consider is the contrast ratio- a...intensity, possible excitation lasers) it is also important to optimize your activation protocol for your ...photoactivatable fluorescent proteins Some of the most important applications for PA-FPs involve enhancing optical...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic cargo can be customized...delivery methods include focused ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular...specific tissues and cell types. If desired, spatiotemporal control of transgene expression or activity...effective in both mouse strains. It is therefore important to review the literature for use of a capsid in...thank Viviana Gradinaru and Tim Shay for their support and input. Resources and references Recommended... The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Molecular Therapy...expression from mouse to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754...

  13. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...and PI, it’s easy to miss out on beneficial opportunities or to find oneself unsure of the next steps....planning meetings with each trainee to create a supportive, growth-oriented lab culture. The aim of these...the overall tone of the meeting is almost more important. As DePace says, science is full of “terrible ...postdoc training. On the other side, the PI has an opportunity to check in with lab members, determine how to...

  14. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding...Since transformation efficiency is inversely proportional to size, and many plant scientists want to transfer... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ... Agrobacterium and infected plants. Further opportunities with pSiM24 While these properties of pSiM24...

  15. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...Zygosaccharomyces bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

  16. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...make sure everyone in the meeting has an equal opportunity to contribute. Using video conferencing tools...scientists working in different time zones, it’s important that everyone can access data and drafts of manuscripts...sure there is a point person responsible for incorporating everyone’s suggestions into a final version ... spent time in each other’s labs. This is an opportunity for the researchers involved to learn techniques...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...behaving mice to gather the following types of data: Report time-course and concentration of released DA in...optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling during learning and ...moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian...

  18. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...Improvement of Cowpea, a knowledge database for this important agricultural plant. Her current research focuses...proteins in order to manage insect pests and to support sustainable agriculture. For this project the expression...in Mexico. At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

  20. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ...hands-on science lab kits and digital curricula to support them. Caitlin’s interview is broken into two parts...lifelong hobby for Caitlin Runne-Janczy. “I wrote reports for fun in fourth grade because I was weird like...hands-on science lab kits and digital curricula that support these kits, is the perfect blend of these two passions...actually stumbled upon, about 6 months in, this opportunity to do scientific editing.” It was for Cactus ...
Showing: 261 - 280 of 781 results