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Addgene

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We narrowed to 512 results for: SET

Showing: 261 - 280 of 512 results

  1. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...

  8. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes...

  13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ... a small subset of cells? Other proteins may also be utliized as scaffolds, including the set of red fluorescent...recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription...absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre isn’...

  15. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...

  18. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies...

  19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...
Showing: 261 - 280 of 512 results