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We narrowed to 527 results for: SET

Showing: 261 - 280 of 527 results
  1. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...
  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...
  3. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...
  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...
  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...
  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes...
  7. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...
  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...
  9. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...
Showing: 261 - 280 of 527 results