We narrowed to 610 results for: STO;
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TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy...about some of SITN's other initiatives, email sitnboston@gmail.com. Many thanks to our guests, Vini Mani...
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Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist and photographer based at the...gathering momentum, including in Cambridge and Bristol. If you’re a scientist who spends many hours at... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School....pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School.... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m....University of Illinois Chicago Boston Children's Hospital California... Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids from... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m....small molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy...mesothelioma sufferers. This disease is less widely understood and often misdiagnosed. Like other forms of mesothelioma... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover letter...project managment) your resume should also be customized to highlight different skills and to get the ... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific...resulted in complete or near-complete excision of the stop sequence. In contrast, Dre recombinase, the close... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....lentiviral CRISPR library. “This enables you to do customized genetic modification on a scale that was really...Using CRISPR/Cas to Edit Disease Out of the Genome History of CRISPR Cas - A tale of survival and evolution... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....and Resources References Fellmann, C., et al., Cornerstones of CRISPR-Cas in drug development and therapy...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering ... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....the fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend...and references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only downloadable...scientists 👨🏽🔬👩🔬! We built this so you could stop struggling to make figures in PPT 🔬 Also, the base... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...range of the ~1011 commensal bacteria per gram of stool for humans. Bourdaeu et al also used arg1 to in ... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are...restriction enzymes, but you will also be able to create custom annotations and design additional experiments like... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....sharing services, as well as #happydepositors who stopped by to say hello. Needless to say we all got very...the conference and talked to some scientists who stopped by the booth about amazing work already represented... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....selectively remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is ... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....interest for which you have several antibodies in stock. Why are there so many options for a single target...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS cells. They then showed that...frankenbody probe could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m...., 2015). At its very core, peer-review is a touchstone of the scientific method, ensuring that published... and Records: The Royal Society Journal of the History of Science 69.3 (2015): 337-352. PubMed PMID: 26495581... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....endocytosis inhibitor sucrose. Previous work by Stoneham et al. demonstrated that the entry of AAV2 into...157.1 (2012): 228-236. PubMed PMID: 22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou...