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We narrowed to 738 results for: TRI

Showing: 261 - 280 of 738 results

  1. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...solution. Proteins and genomic DNA are removed by centrifugation. At this point, the plasmid DNA sample can ...binding DNA, the glass particles are pelleted by centrifugation and washed before DNA is eluted with water ...was free of agarose and could be digested with restriction enzymes.  Cellulose nucleic acid purification...phosphoric acid for 3 minutes followed by a short centrifugation for a total of four times before a wash with...

  2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...decided this protocol would be a worthy first contribution to the protocols.io community. Did I mention... protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial transformation... was created in 1999 and allows scientists to contribute protocols or provide links to protocols on their...subscription-based software, Labguru does offer free trials and pricing options for academic labs, departments...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...new AAV approach using regulatory elements that restrict expression to this cell type. To this end, they...the size of DNA fragments. Depending on which restriction enzyme is used to cut the plasmids encoding the...are shared through Addgene without licensing restrictions. Use these instructions to prepare the PSU ... of telomere length regulation called telomere trimming and have proposed a role for TZAP, a Kruppel-like...regulating the upper-bound of telomere length. Telomere trimming can be assayed by looking for the production of...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...made great strides to map brain regions and allocate specific functions to them. Electrical recordings...circuit by integrating neuronal signalling and triggering release of the stress hormone norepinephrine ...levels in general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize ...et al., 2017). They characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...community. Note: Cary Valley and Mary Gearing contributed to the update of this post. References 1. Abudayyeh...

  6. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...or G) is supplied as a dideoxyribonucleoside triphosphate (ddNTP) so that after its addition, DNA synthesis...attached to the flow cell membrane and have an electrical current running through them. DNA or RNA is pulled...each nucleotide passes through the nanopore, the electrical current recorded by the instrument changes. Base-calling...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...protein ticker tape Teaching an old antibody new tricks   New compact, efficient prime editors By Susanna....10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While IHC protocols typically...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...antibodies in Addgene’s collection since we began distribution and has been verified to perform well in standard...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...envelope proteins from another virus to either restrict or broaden the host cell range (tropism). Pseudotyping...vesicular stomatitis virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer..., Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...and last updated Aug 12, 2021. This post was contributed by guest bloggers, Yiwen Zhang, a Ph.D. candidate... pTargetF with the ccdB gene containing BsaI restriction enzyme sites at its ends to generate pEcgRNA....that is 2.6 times higher when compared to the industrial strain. In our metabolic engineering case study...high-yielding strain which had 6 times the yield of the industrial strain. Not only is MUCICAT better, it is faster...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes ...techniques are currently being tested in clinical trials to detect mesothelioma earlier than ever. From ...been banned in the United States and many other countries, and it is still imported, exported, and used ...from this dangerous material. This post was contributed by the Mesothelioma Cancer Alliance. Additional...

  11. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...pair of restriction enzymes. These enzymes, Nt.BbvCI and Nb.BbvCI, recognize the same restriction site, ... be sense. Does the orientation of the BbcVI restrict site matter? Most of the time no, but there are...directions, you just need to use a plasmid with BbvCI restriction sites in the same orientation as the gene or ...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...is far from perfect. One of the most limiting attributes of rats is their difficult to manipulate genome...making them useful for in vivo studies. These attributes make frog eggs some of the most useful tools ...utilization, Arabidopsis has made significant contributions to understanding plant and crop science alike...). E. coli as a Model Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net...

  13. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv. PLoS...there are many scFv-based therapies in clinical trials or already in the clinic (Bates and Power., 2019... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

  14. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...building and testing new products or methods, using metrics and feedback, taking a look at what works and what...partially reopened. But there were still many restrictions in place, most importantly, masks.  Besides ...Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The lab re-opened...very early in the pandemic with some incredibly strict safety protocols to keep Addgenies safe. Space ...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...type and laser voltage. The voltage describes an electric potential that can be applied to the photomultiplier...photomultiplier inside the cytometer to increase the electric current and thereby the signal strength. So, we...dimensions displayed in the plot. (B) A more restrictive gate results in fewer cells being considered ... personal preference, some people like to make stricter gates while some like to make looser ones (Figure...

  16. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...Addgene to accelerate biomedical research by distributing antibodies developed by IPI. Our integrin antibodies...

  17. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... be captured on streptavidin affinity beads or matrices. The bait protein can even target BirA* to specific...identify the proteomic map of the human mitochondrial matrix (Rhee et al., 2013) and determine the topology ...of mitochondria in living cells via spatially restricted enzymatic tagging." Science 339.6125 (2013): ...Biotinylation Other resources Proximity labeling review: Trinkle-Mulcahy, L. (2019). Recent advances in proximity-based...

  18. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute...researchers with a valuable tool for understanding the contribution of phosphorylation to enzyme function. The development...recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA synthetase...-through translation of many stop codons and detrimental effects on cell growth. The C321.ΔA strain was...

  19. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...This post was contributed by Damien Wilpitz founder and lead consutant at Experiment Designs Consulting...funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive...all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools...they don’t have the money. If the department has a strict budget, or a lack of resources, then ask for suggestions...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst. While... 2013, Figure 1. Used under Creative Commons Attribution License. Within the nervous system, remember... 2019, Figure 4. Used under Creative Commons Attribution 4.0 International License.   Inducible promoters...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic...
Showing: 261 - 280 of 738 results