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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 487 results for: Vit

Showing: 261 - 280 of 487 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... situ. As a result, they had to be expressed in vitro before the bacteria were injected, limiting their... Use a subset of the cells to verify the mARG positivity, which can be observed under phase contrast microscopy...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...fusing mutants of Cas9 deficient in DNA cutting activity to a transcriptional activation domain (Fig 1)...Worry less about off targets Unlike Cas9 cutting activity, off target effects are generally not regarded...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...using microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within...used to measure conformational changes, cleavage activity using FRET-based biosensors, and interactions ...

  5. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...have sufficient on-target and very low off-target activity, but should also mediate a DSB at optimal distance... the desired zygosity. Furthermore, re-editing activity of the guide RNA should be minimized by incorporating...

  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...protocols. To facilitate this, some ELNs allow you to invite people and assign them roles and permission that...in handy if a supervisor wants to see the full activity on a project. Can you meet the ELN support team...

  8. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...indirect labeling is through antibody host species reactivity. Just like for western blotting, a primary epitope-targeting...biotin-streptavidin binding and antibody host species reactivity. When sorting cells with a high resolution for...

  9. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...vector that enables protein expression in any in vitro translation system. The gene set was carefully chosen...been used to produced recombinant proteins for in-vitro assays: Koch, Daniel, et al. "A pull-down procedure...

  10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical...off-target cleavage. In both cases, translating the in vitro work into an animal model comes with two key hurdles...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...from pLac repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated...increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced...

  12. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product as a template... design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after editing, make...

  13. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19...No photoexcitation High Ion specificity High sensitivity Easy expression Gives ratiometric data The result...

  14. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...specificity control, label control, and cross-reactivity control when multiplexing. A, wild-type and knockout... included to uncover secondary antibody cross-reactivity. Non-specific binding  Primary antibody specificity...

  15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by species and route ....2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong, A. C. H., Gokal, D., Feng, Y., Tabar...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...genome to be probed for proteins with host-binding activity without the need for genome sequencing or annotation...nitrocellulose for subsequent probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of...

  18. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...Plus, just one role can encompass many of these activities leading to a rich day-to-day work life.  Action...professional societies, and industry through convening activities, formal lobbying, or strategy setting.  The “...

  19. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...core goals of antibody validation:   Confirm reactivity with the target antigen - Recognition of an immunogen...methods. These approaches have relatively low sensitivity, which can be problematic if you are trying to...

  20. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...have allowed for real-time monitoring of neuronal activity across substantial 3D volumes. In addition to ...Weijian, and Rafael Yuste. In vivo imaging of neural activity. Nature Methods 14.4 (2017): 349-359. PubMed PMID...
Showing: 261 - 280 of 487 results