We narrowed to 520 results for: emb
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TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....findability from multiple angles We assembled a cross-team team, with members from the Customer Service, Data...
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7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....pooled library In a paper published in Nature in December, Konermann and her colleagues in Feng Zhang’s ...was on the right track. The next insight was to assemble a synthetic transcription activation complex consisting... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog PostPublishedAug. 7, 2014, 1:58 p.m....you give someone a card they are more likely to remember you if you follow up and if you give someone your... online to follow up with in person. Email lab members that have moved on to see if they will attend. ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028 Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m..... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m...., the “Reproducibility for Everyone” team was assembled when it was realized that interactive workshops...connected, this diverse and international team assembled the right people at the right time. Be inclusive... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m.... lab members. We can set ourselves up for success by giving explicit instructions to lab members through... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....something that is actually useful to you. When embarking on a new literature search, try to find a recent... rudimentary, but it works for me. I can often remember which paper I need to open, but when I can’t it... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone to begin considering...papers, published in Molecular Systems Biology in November, Voigt and Alex Nielsen describe what’s possible... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....variants: Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy...2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https...