We narrowed to 535 results for: epo
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TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m.... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...ubiquitous promoter drives expression of an mCherry reporter. After using mCherry for FACS sorting of the ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m.... fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...Purification Protocol Video rAbs in the Addgene repository... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m.... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....antibodies expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the... VH and VL sequence. These plasmids have been deposited with Addgene for distribution to the scientific... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....ability to infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....s take a look at a few examples with plasmids deposited at Addgene. PRESTO-TANGO: The ligands that activate...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m.... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m.... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...open science, Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....research. Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe the...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this...