We narrowed to 632 results for: ncl

...of physical materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ...materials and methods section have been updated to include the option of citing with resolvable compact identifier...

...symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery... promising clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic...

...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

...plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested with...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year.... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

...Reis e Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ...

...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

...information on each cancer clinical sequencing report includes rare variants that haven’t been characterized?...the scientific community through Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding...

...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

...Other examples of chemically induced promoters include positive inducible alcohol and steroid regulated... be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90-derived...

...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

...shares many common features with gamma-retrovirus including genome architecture, host integration, and its...its cargo, in fact its viral life cycle does not include genomic integration. While the expression of adenovirus...

... tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody ...

...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

...controls are used in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...

...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

...The information is organized in wiki format and includes biochemical properties, previously established...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

... is not fluorescence! Chemiluminescence, which includes all bioluminescence, is the production of light...Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which...

...organisms in which it was previously not an option, including Drosophila and C. elegans.   Find the TurboID ...that one might perform using the purified domains include protein stability, small molecule binding screens...