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Addgene
Showing: 261 - 280 of 619 results

  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

  3. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...controls are used in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...

  4. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...The information is organized in wiki format and includes biochemical properties, previously established...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

  5. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

  7. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Other examples of chemically induced promoters include positive inducible alcohol and steroid regulated... be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90-derived...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...sociology, behavior, and artificial intelligence (including a team that created a novel form of AI that can...require large data sets. Whether these data sets include videos of behavior, many many images of brain regions...

  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

  12. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... DNA there, although other restriction sites (including other XbaI sites not preceded by GA or followed...either be difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes...

  14. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...intact β-galactosidase produces pigment from x-gal (included in the transformation plate medium), turning the... colony blue. We should note that IPTG is also included in the medium to ensure transcription of the lac...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide...

  16. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...proteins that are found in and on everything, including your skin. Here are some general tips for working...degrade RNA. Keep a clean work area, which may include spraying your bench down with a product to get ...

  18. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

  19. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...pillars for antibody validation.  The 5 pillars include genetic strategies that test expression in knock-out...conditions. Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get...

  20. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...
Showing: 261 - 280 of 619 results