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We narrowed to 400 results for: organization Addgene

Showing: 261 - 280 of 400 results
  1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...
  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...interest. This is a particularly nice feature as organ-specific injections, or the location of a specific...
  3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...
  4. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...
  5. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...want to amplify sequences from a large genome organisms like mouse or human. There are several online ...currently the product development scientist at Addgene. In the lab he develops new assays and processes...possible aspects.     Additional Resources on the Addgene Blog Browse our Plasmids 101 blog posts Read blog...about different plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our ...
  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...in Molecular Biology. This annual symposium is organized by international PhD students at the Max Planck...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study...
  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing of all transcripts gives...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...
  8. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ... a repository others could request from — AddgeneAddgene was established as an e-commerce company, ...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ...young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's 10 year anniversary...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning ...
  9. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...helping researchers visualize the genome and its organization within the nucleus, also called the 4D nucleome...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic...PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling...PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of...PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering...
  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...careers through structural change in research organizations. “Fix the Knowledge” or “gendered innovations...Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How Scientists Are Trying to Shape ...
  11. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different...AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview of AAV Encoded Calcium Biosensors.... DOI: 10.3389/fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service...
  12. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...embryos and adult fish. Zebrafish are an ideal model organism for these experiments because developing embryos...PubMed PMID: 16369538. Additional Resources on the Addgene Blog Read Other Optogenetics Blog Posts Sign Up...about Small Molecule Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check...
  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving platforms...shows workflow of open hardware.    Conclusion Addgene is a great example of how sharing of data and molecular...
  14. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ....1038/s41580-021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...
  15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ... specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able to...PMCID: PMC6255809.  Additional resources on the Addgene blog Read our CRISPR 101 blog posts Find more ... Download the CRISPR 101 eBook Resources on Addgene.org Visit our CRISPR plasmids and resources page...
  16. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...Additional Resources on the Addgene Blog Volunteer to write for the Addgene blog Listen to Our Podcast...Communication Posts Resources on Addgene.org Explore careers at Addgene Other Resources The National ...government. All the Health and Human Services (HHS) organizations have so many [science communication] needs. ...
  17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...illuminate cell organization." bioRxiv (2017): 123042. Additional Resources on the Addgene Blog Learn More...with GFP. These plasmids are available through Addgene, and the stem cell lines we’ve made using them ...a variety of endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...
  18. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... of your organization Time management Managing a team Leadership Resources on Addgene.org Check out...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...choose.    Resources Additional resources on the Addgene blog More from the transferable skills guide: ...
  19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...choice for many behavioral studies, though other organisms such as rats or zebrafish are also used. With ...detail and share ways how scientists have used Addgene’s plasmid repository and viral service for their...PMCID: PMC4135440.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...
  20. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...career offices, postdoc offices or professional organizations can provide referrals. There are bad coaches...suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't...investment in your future. Additional Resources on the Addgene Blog 6 Steps to Submitting a Resume That Gets ...Future of Research Symposium Resources on the Addgene Website Check out Our Career Page for Recent Job...
Showing: 261 - 280 of 400 results