We narrowed to 793 results for: por

...regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional analysis of proteins... by an increase in the size of the lower band (portion of the plasmid containing the mutation). Similar...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...you can simply amplify and mutate this smaller portion of the plasmid with a primer pair that generates...

...this article, we’ll go through some of the most important considerations to make when planning and performing...same sample. For this strategy to work, it is important to ensure that you are using secondaries with ...primary, so we’ll start there. Some of the most important considerations are to make sure your secondary...publications or as a review on a company website. It is important to review the data and protocols available for...regardless of how “risky” they may be, it is always important to verify their performance in your hands and ...

..., documents need to minimize jargon. Equally importantly, you really are selling yourself. A sales pitch...anyone could relate to, that people would agree is important to understand. (1-2 sentences). For example: Distinguishing... lot of time to discuss and the questions are important. However, it's much easier to focus on the questions...version of my manual, I added new chapters to incorporate the experiences of the people that provided me...interviews. We didn't have Skype in 2004. I also incorporated example documents. This part has generated a...

...Massachusetts Nonprofit Day, which was the perfect opportunity to test shooting over Zoom, everyone’s new favorite...directing. Everyone’s schedules were busy, so it was important for me to let people record at their leisure. ... were still many restrictions in place, most importantly, masks.  Besides the muffled voices beneath masks...out of the way when it was blocking some more important lab work from being done. You can watch the first...things return to relative normalcy, I’ll be incorporating that mobile monitor stand into every shoot. ...

... This is important to ensure that you’re looking at institutes that have the means to support your work...screening out irrelevant candidates, and, more importantly, it’ll help you define salary costs. 4. Ascribe...make these decisions in the following order of importance: 1. First make decisions based on experimental...and availability of the equipment is of utmost importance. Conducting a successful lab start up negotiation...

...methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes... mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes...Allows uptake of large plasmids hee   High electroporation efficiency supE44 (glnV44)   Suppression ...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F-mcrA Δ(mrr-hsdRMS-mcrBC...propagating plasmids expressing the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ...

... homology arms and a selection marker, then electroporate it into mouse embryonic stem cells. After selecting...compatible with standard microinjection and electroporation methods using in mouse transgenics. Designing...between the ampulla and infundibulum. In vivo electroporation is performed immediately after injection. Image...tweezer-type electrodes are placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...

...engineer cellular processes that are influenced by temporally ordered biochemical events. How do RSMs work?...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...

...function, but these connections are exquisitely important in the brain. To better understand how astrocytes...extracellular matrix proteins, cell adhesion molecules, transporters, ion channels, GPCRs, and more.  Figure...Grelet Lab developed MitoTRACER, a retroviral-based reporter system that permanently labels the cells receiving... and signal response upon Ca2+ binding. Most importantly, it doesn’t display the problematic photoswitching... using an anti-GFP nanobody (Figure 4). Once incorporated into a condensate, the killswitch disrupts its...

...injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et al., 2014), and postsynaptic ... system, remember that some serotypes can be transported retrogradely and/or anterogradely along the axon...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse...Favre, D., Blouin, V., Provost, N., Spisek, R., Porrot, F., Bohl, D., Marmé, F., Chérel, Y., Salvetti,...

...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ... drove work in countless other systems, whereas import of CRISPR technology into C. elegans has been a...

...editing technology led to the development of many important medical and agricultural products. But it also...Ridley Scott wonder what would happen if these corporations would extrapolate genetic engineering to exploit...which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases In 1989 the...SciFi is one of the most imaginative and most important genres of literary fiction. It has always served...health sciences, energy, and agriculture, its portrayal in popular fiction has been largely negative. ...

...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...provided by the studies in Tsien lab, covered a portion of the color spectrum that GFP and its derivatives...reach. Nobel Prize for fluorescent proteins The importance of fluorescent proteins was recognized in 2008...

...looking through a paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash...itself when looking down a paper thin and very portable microscope. Less fluorescence admittedly, but ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...

...number of cells needed is lower, and perhaps most important, targeting all genes doesn’t necessarily add value...cytokine known to cause the up-regulation of an important surface protein (e.g. PD-L1 on a cancer cell)....are migratory, and their exact localization is important for their function. Their migration is induced... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

...impossible to determine specific protein turnover or temporal expression using standard FP-chimeric proteins...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...localization, or leading to protein aggregation. Another important parameter to consider is the contrast ratio- a...intensity, possible excitation lasers) it is also important to optimize your activation protocol for your ...photoactivatable fluorescent proteins Some of the most important applications for PA-FPs involve enhancing optical...

...transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic cargo can be customized...delivery methods include focused ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular...specific tissues and cell types. If desired, spatiotemporal control of transgene expression or activity...effective in both mouse strains. It is therefore important to review the literature for use of a capsid in...thank Viviana Gradinaru and Tim Shay for their support and input. Resources and references Recommended... The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Molecular Therapy...expression from mouse to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754...

...and PI, it’s easy to miss out on beneficial opportunities or to find oneself unsure of the next steps....planning meetings with each trainee to create a supportive, growth-oriented lab culture. The aim of these...the overall tone of the meeting is almost more important. As DePace says, science is full of “terrible ...postdoc training. On the other side, the PI has an opportunity to check in with lab members, determine how to...

...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding...Since transformation efficiency is inversely proportional to size, and many plant scientists want to transfer... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ... Agrobacterium and infected plants. Further opportunities with pSiM24 While these properties of pSiM24...

...Zygosaccharomyces bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...