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We narrowed to 644 results for: rest

Showing: 261 - 280 of 644 results

  1. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...expression. Cultures are then lysed and the protein of interest is purified using an affinity tag. Additional ...Determining the best way to express one’s protein of interest can save a lot of time later. Protein purification...C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below), enabling...

  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... to on Twitter? Prior to writing this post, my interest in Twitter was fleeting. I’ve had an account for...Unfortunately, it never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because... scientist? If you don’t use Twitter, are you interested in getting started? Reply in the comments below...

  3. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult...plasmids and publications relevant to your research interests. We do this by promoting citation, supporting ...encourage you to fill out this form if you’re ever interested in writing your own blog post about a plasmid...

  4. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...to determine which job boards list positions interesting to you. Many job postings will result in hundreds...address how you are qualified and why you are interested specifically in the position. We frequently receive...cover letters. It is also important to express interest, even excitement, in a position. Everyone prefers...

  5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...somewhat unusual things. For example, I am not that interested in meeting movie stars, but when I met the astronaut...a scientist up there, but the panelists were interesting enough to make up for the somewhat naïve questions...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...

  6. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...NEBCutter2: For finding restriction enzyme cut sites Webcutter 2.0: For finding restriction enzyme cut sites ... different types of cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion...

  8. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA....“vectors” or “constructs.” To insert a gene of interest into a vector, scientists may utilize one of a... a variety of cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning...

  9. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – large tracts...like YNP thermal sites - are also of practical interest since they’ve previously yielded valuable enzymes...bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number of steps in ...

  10. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward - and this ... the pieces that make you think, “Oh, that’s interesting! How did you solve that problem?” Those are the...your audience in with something that sparks their interest and makes them want to know more. This could be...

  11. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...collection, you'll likely find some proteins of interest for which you have several antibodies in stock...another, or if you’re looking for a protein of interest while it’s in a transition state or specific configuration... to validate that it recognizes the protein of interest in specific configurations or conditions. In those...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...several excellent guest posts from our depositors. Interested in writing your own post for the Addgene Blog...(AAVs) are commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are ...customizable functions to your DNA sequence of interest. Potential applications of the system include ...

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...plasmid that produces biotin ligase and a protein of interest fused to maltose binding protein (MBP, New England...-Avi-His/BirA to add biotin to your protein of interest Grow fusion protein plasmid in NEB T7 SHuffle... Vanderbilt Eye Institute and is particularly interested in cell signaling.     Additional Resources on...

  15. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...clone 500-700 bp homology arms for your gene of interest into the FP-SEC vector using Gibson assembly. ...as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel...

  16. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning projects should be fun and...researchers. We have been excited to see so much interest, because it means more scientists will be working...Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science, and making...

  18. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...determine if the fluorescent proteins you are interested in are compatible with your microscope optics...related protein, if you know that your protein of interest has a similar spectrum. For example, here's a ...need to try several proteins in your system of interest. Finally, in this set of proteins the green and...

  19. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...the bacteria can easily uptake your plasmid of interest. Scientists can also use electroporation, the ...scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s genome. To do...create both transient cell lines, where a gene of interest is expressed but not integrated into the genome...

  20. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...been hampered by the need to tag each protein of interest and, for in vivo work, generate a dedicated transgenic...GFP-nanobody rather than a specific protein of interest. This fusion protein can then be co-expressed ...expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target...
Showing: 261 - 280 of 644 results