...current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “...

...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

...its surroundings and being shaped by them. Another set of highly influential microenvironment cells are ...

...deposit to Addgene. We are also lauded by another set of stakeholders - the tech transfer community. In...

...“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved...

...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

...solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly...

...when thinking about crafting your own poster. What sets a scientific poster apart? First, a good poster ...

...connections This is a site for meeting people. Use the settings to let people see who you are when you look at...

...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

...tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can ...

... a small subset of cells? Other proteins may also be utliized as scaffolds, including the set of red fluorescent...recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription...absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre isn’...

...for this transition. Second, a coach will help you set goals and then hold you accountable for carrying ...

...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies...

...case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...

...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...

...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...

...sample, we’ll then load it on to our new MiSeq and set up the run making sure to note the appropriate barcode...

...happens when the antibody is produced from a new set of animals), and wrong application (different experiments...