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... not known why or how this happens. Therefore, I set out to study how protein turnover is regulated in...and specific antibody and the proper microscope setup we can observe the whole population of one particular...

...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of...

...for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

...your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF...

...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...

...tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can ...

...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing...

...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

...solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly...

...when thinking about crafting your own poster. What sets a scientific poster apart? First, a good poster ...

...connections This is a site for meeting people. Use the settings to let people see who you are when you look at...

...“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved...

...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

... range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes...

...its surroundings and being shaped by them. Another set of highly influential microenvironment cells are ...

...deposit to Addgene. We are also lauded by another set of stakeholders - the tech transfer community. In...