...an important role in viral entry, which are inhibitors of SARS-CoV-2 entry and become upregulated during...plasmids, with many available to scientists in industry. Since March 2020, we have received over 13,000... which human genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al....glad to join these efforts and help academic and industry scientists through plasmid sharing and more. As...

...create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used... various constructs with applications in flow cytometry approaches in mammalian cell culture systems (...deoxyribonucleic acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780...Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015...deoxyribonucleic acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810...

... contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do ...guest blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing...progress and prospects." Journal of Biological Chemistry 284.26 (2009): 17365-17369. PubMed PMID: 19329793...ligase reaction and bead enrichment." Analytical biochemistry 434.1 (2013): 34-38. PubMed PMID: 23142428. ...of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed PMID: 24328157. 21...

...application — flow cytometry, western blot, immunohistochemistry, immunocytochemistry, ELISA — but in all...antibody data report, showing the results of an immunocytochemistry experiment performed with the anti-Lhx6.1...

... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple... seminars, such as those in bioinformatics or chemistry, even if your primary focus lies within a biological...successful research career, whether within academia or industry. Albert Einstein once said: “There are only two...

...Fighting with antibodies to produce immunohistochemistry images that are crisp, bright, and lacking in...mouse-on mouse” (or any “species on species”) immunochemistry (IHC) applications an issue? The culprits are...avoiding background secondary binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your...impact other techniques like Western blots or immunocytochemistry (ICC). Not all of the solutions described...

... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple... PhDs face when transitioning from academia to industry is whether to stay hands-on in the lab or pivot...exploring alternative career trajectories within the industry, but the transition wasn’t easy - I had to reinvent...

...am heading into my third year of college as a biochemistry major and a Spanish minor, it’s time for me ...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...the Addgene blog. A few of my friends in the Biochemistry major seem to have the idea that if you don’...of Business Development, got her PhD in Plant Biochemistry in France. When she moved to the U.S., she discovered...

...validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important to note that...compatible with your conjugate and conjugation chemistry.  Quantification Once you’ve collected your antibody...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ...

...used rAAV FLEx-based vectors to study the neural circuitry behind the mechanisms of sleep and anesthesia....Cre recombinase protein." Journal of Biological Chemistry 259.3 (1984): 1509-1514. PubMed PMID: 6319400....behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157. PubMed PMID... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

...immunoprecipitation, immunohistochemistry, immunocytochemistry, and flow cytometry. It's a great introduction...

...a desired component are cloned into Golden Gate entry vectors via traditional restriction enzyme cloning...cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden Gate cloning step to...to generate the final construct. Up to eight entry vectors can be used for each Multisite Gateway compatible... Production group at Max-Planck Institute of Biochemistry has developed a number of expression vectors..., and plant resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination...

...genetic circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic...shuttle, our driver regaled us with tales of back country skiing, fly fishing, and local grizzly bear attacks...

... science began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with...do,” Stegmeier says He began this journey in biochemistry made the foray into genetics during graduate... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...

... like serotype, promoter, or tool type and each entry contains an image along with key experimental details...to input your data for sharing on the site. The entry form has 5 general question types and it takes approximately...use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment...

...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...uses it to match qualified scientists to jobs in industry, academia, and government. This great resource... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...

...resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015). Nevertheless, the use of these...visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed ... allowed for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al....PMID: 21825165. PubMed Central PMCID: PMC3174680. Buttry, J.L., and Goshgarian, H.G. (2015). WGA-Alexa Transsynaptic...

...applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting...

...industries tend to hire their former interns to fill entry-level positions right out of college. Prior to March...also serve you well when you are applying for an entry-level position following graduation. While these...for internships, scholarships, fellowships, and entry-level positions post graduation. Many thanks to...

...unique regions as well, though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate... from the transfer plasmid. Viral packaging and entry The viral packaging proteins for AAV are rep and...replication of the dsDNA virus. Viral packaging and entry Adenovirus is quite large and has more helper plasmid...